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Updated: Dec 8, 2025

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq
Published on: July 10, 2019
New developments in RiPP discovery, enzymology and engineering
Manuel Montalbán-López1, Thomas A Scott, Sangeetha Ramesh
1Department of Microbiology, University of Granada, Spain.
Ribosomally-synthesized and post-translationally modified peptides (RiPPs) are diverse natural products. Recent advances have significantly improved understanding of RiPP biosynthesis, engineering, and discovery using bioinformatics.
Area of Science:
- Natural Product Biosynthesis
- Biotechnology
- Bioinformatics
Background:
- Ribosomally-synthesized and post-translationally modified peptides (RiPPs) represent a vast class of natural products.
- A 2013 review highlighted commonalities in RiPP biosynthesis and potential for bioengineering and genome mining.
- Significant progress has been made since 2013 in understanding RiPP assembly and applications.
Purpose of the Study:
- To review recent advancements in the field of RiPPs since 2013.
- To discuss new RiPP classes, post-translational modification mechanisms, and enzyme-substrate recognition.
- To cover genome mining tools and engineering strategies for RiPPs.
Main Methods:
- Literature review of developments up to June 2020.
- Analysis of new RiPP classes and biosynthetic pathways.
- Discussion of bioinformatic tools for RiPP discovery and engineering strategies.
Main Results:
- Discovery of novel RiPP families.
- Enhanced understanding of primary and secondary post-translational modifications.
- Improved insights into leader peptide recognition by modifying enzymes.
- Advancements in genome mining and RiPP engineering.
Conclusions:
- The field of RiPPs has experienced substantial growth since 2013.
- New discoveries and improved methodologies continue to expand the potential of RiPPs in various applications.
- Future research directions include further exploration of RiPP diversity and bioengineering applications.
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