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Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity

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Distinct T-cell immunophenotypic signature in a subset of sarcoidosis patients with arthritis

Avinash Jain¹, Harshit Singh¹, Alok Nath¹

¹Department of Clinical Immunology and Rheumatology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India.

The Journal of the Royal College of Physicians of Edinburgh

|September 16, 2020

Abstract

Background:

The objective of the study was to assess T-cell subsets in sarcoidosis patients with or without articular involvement.

Methods:

Treatment-naïve patients were divided into Group A (articular) and Group B (non-articular) based on joint involvement. Flow cytometric analysis of T-cell subsets and pro-in˜flammatory cytokines were carried out in the peripheral blood.

Results:

Patients in group A (n = 29, mean age 40 ± 10.1 years) were compared with group B (n = 18, 43 ± 12.2 years). T-cell subsets: the CD4/CD8 ratio was abnormal in two groups but had no significant difference (p = 0.63). Ratios of Th1/Treg, Th2/Treg and Th17/Treg were significantly increased in group A as compared to group B [p < 0.001] indicating polarisation of T-cell subsets. CD8 T-cells in group A had higher granzyme B expression (p = 0.03). B cells were increased in group A [p = 0.04]. Ratio of IFN-γ /IL10, IL-4/IL10, IL-17/IL10 in sera as well as culture supernatant were significantly higher in group A as compared to group B.

Keywords:

cytokines granulomatous disease joint musculoskeletal non-tubercular t-cell phenotypes t-regulatory cells ﬂow cytometry

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Keywords:

cytokines granulomatous disease joint musculoskeletal non-tubercular t-cell phenotypes t-regulatory cells ﬂow cytometry

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