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Updated: Dec 8, 2025

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Published on: October 31, 2020

Super-Resolution Spatial Proximity Detection with Proximity-PAINT.

Florian Schueder^1,2, Juanita Lara-Gutiérrez^2,3, Daniel Haas²

¹Faculty of Physics and Center for Nanoscience, LMU Munich, Geschwister-Scholl-Platz 1, 80539, Munich, Germany.

Angewandte Chemie (International Ed. in English)

|September 16, 2020

Summary

This summary is machine-generated.

We developed Proximity-PAINT (pPAINT), a super-resolution microscopy method to visualize biomolecular interactions. This technique enhances detection sensitivity and accuracy for studying cellular life at the molecular level.

Keywords:

DNA-PAINT Protein-Protein interactions Proximity detection Single Molecule Imaging Super-resolution microscopy

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Area of Science:

Molecular Biology
Biophysics
Microscopy

Background:

Understanding cellular life requires visualizing biomolecular interactions.
Spatial proximity is a common indicator of direct biomolecular interaction.
Existing visualization methods have limitations in resolution, dynamic range, or sensitivity.

Purpose of the Study:

To introduce Proximity-PAINT (pPAINT), a novel super-resolution microscopy technique.
To overcome limitations of current methods for visualizing spatial proximity of biomolecules.
To enable sensitive and accurate detection of molecular interactions in cells.

Main Methods:

Developed Proximity-PAINT (pPAINT), a variation of DNA-PAINT super-resolution microscopy.
Utilized a split-docking-site configuration for proximity detection.
Benchmarked and optimized pPAINT using designer DNA nanostructures.

Main Results:

pPAINT achieves high sensitivity and low false-positive rates.
The detection distance in pPAINT is tunable.
Demonstrated cellular applicability by visualizing alpha- and beta-tubulin proximity in microtubules.

Conclusions:

pPAINT offers a sensitive and versatile tool for visualizing biomolecular spatial proximity.
The technique advances super-resolution microscopy for molecular-scale cellular studies.
pPAINT provides new insights into the functional interactions of biomolecules within cells.