Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Non-Invasive Brain Targeted Delivery of Cannabidiol for Alleviating Neuroinflammatory Disease.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Correction to: Lipopolysaccharide and Morphine-3-Glucuronide-Induced Immune Signalling Increases the Expression of Polysialic Acid in PC12 Cells (PMID- 31646464).

Molecular neurobiology·2026
Same author

Two-dimensional NIR-II AIE nanotheranostic probes with ultralarge Stokes shifts for surgical navigation and ablation of glioma.

Science advances·2026
Same author

High-Throughput Generation of Tumor Spheroids via Droplet Microfluidics for siRNA-Loaded Nanomedicine Assessment.

Advanced healthcare materials·2026
Same author

Nanoparticle-mediated targeting chimeras transform targeted protein degradation.

Nature nanotechnology·2026
Same author

Comprehensive analysis of human hepatotoxicity and mechanism of the plasticizer dimethyl phthalate.

Naunyn-Schmiedeberg's archives of pharmacology·2026
Same journal

Higher-Order Clustering of Receptors Real-Time Projected by Plasmon-ruler on the Single Live Cell.

Nano letters·2026
Same journal

Achieving Fermi-Level Depinning and Ideal Metal Contact in <i>β</i>-Ga<sub>2</sub>O<sub>3</sub> Devices via MXene Integration.

Nano letters·2026
Same journal

AI-Assisted Electron Microscopy in Structure-Performance Analysis of Advanced Catalysts: From Atomic Resolution to Statistical Significance.

Nano letters·2026
Same journal

Electrically Switchable Ultraslow Dispersionless Polaritons via Twist Engineering in van der Waals Heterostructures.

Nano letters·2026
Same journal

Correction to "Ultrasonication-Triggered Ubiquitous Assembly of Magnetic Janus Amphiphilic Nanoparticles in Cancer Theranostic Applications".

Nano letters·2026
Same journal

Tunable Proximity Valley Splitting Via Interfacial Exchange Pinning in WSe<sub>2</sub>-CrBr<sub>3</sub>-CrPS<sub>4</sub> Heterostructures.

Nano letters·2026
See all related articles

Related Experiment Video

Updated: Dec 8, 2025

Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts
10:33

Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts

Published on: March 8, 2017

8.6K

Light-Emitting Diode Excitation for Upconversion Microscopy: A Quantitative Assessment.

Yueying Cao, Xianlin Zheng, Simone De Camillis

    Nano Letters
    |September 16, 2020
    PubMed
    Summary
    This summary is machine-generated.

    Wide-field upconversion microscopy now uses inexpensive, incoherent light-emitting diode (LED) excitation for single nanoparticle imaging. Time-gated detection overcomes LED limitations, enabling cellular imaging with lanthanide-based upconversion nanoparticles (UCNPs).

    Keywords:
    hydrophilic functionalizationlight-emitting diodesingle-nanoparticle detectiontime-gated luminescenceupconversion nanoparticle

    More Related Videos

    In Depth Analyses of LEDs by a Combination of X-ray Computed Tomography CT and Light Microscopy LM Correlated with Scanning Electron Microscopy SEM
    10:42

    In Depth Analyses of LEDs by a Combination of X-ray Computed Tomography CT and Light Microscopy LM Correlated with Scanning Electron Microscopy SEM

    Published on: June 16, 2016

    9.6K
    An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
    11:20

    An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

    Published on: August 30, 2017

    7.7K

    Related Experiment Videos

    Last Updated: Dec 8, 2025

    Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts
    10:33

    Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts

    Published on: March 8, 2017

    8.6K
    In Depth Analyses of LEDs by a Combination of X-ray Computed Tomography CT and Light Microscopy LM Correlated with Scanning Electron Microscopy SEM
    10:42

    In Depth Analyses of LEDs by a Combination of X-ray Computed Tomography CT and Light Microscopy LM Correlated with Scanning Electron Microscopy SEM

    Published on: June 16, 2016

    9.6K
    An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
    11:20

    An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

    Published on: August 30, 2017

    7.7K

    Area of Science:

    • Nanotechnology
    • Biophotonics
    • Materials Science

    Background:

    • Lanthanide-based upconversion nanoparticles (UCNPs) typically require high-power laser excitation for imaging.
    • Existing methods face limitations due to laser requirements and background noise.

    Purpose of the Study:

    • To develop wide-field upconversion microscopy using incoherent light-emitting diode (LED) excitation.
    • To achieve single-nanoparticle sensitivity with reduced excitation power.
    • To demonstrate cellular imaging applications with UCNPs under LED illumination.

    Main Methods:

    • Utilized a 970 nm LED for incoherent excitation of UCNPs.
    • Implemented time-gated luminescence detection to suppress background noise.
    • Employed polysaccharide coating for hydrophilic UCNP preparation and cellular uptake studies.

    Main Results:

    • Achieved single-nanoparticle sensitivity imaging with LED excitation (1.18 W cm⁻²).
    • Demonstrated effective background reduction using time-gated detection.
    • Successfully imaged cellular uptake of hydrophilic UCNPs under LED illumination.

    Conclusions:

    • Developed a viable method for upconversion microscopy using low-power, incoherent LED excitation.
    • Overcame challenges of LED excitation efficiency and background noise.
    • Paved the way for broader applications of UCNPs in life sciences by reducing reliance on high-power lasers.