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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
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Biotin immunoassay interference: A UK-based prevalence study.

A Sanders1,2, R Gama2,3, H Ashby1,2

  • 1Black Country Pathology Services, The Dudley Group NHS Foundation Trust, Russells Hall Hospital, Dudley, UK.

Annals of Clinical Biochemistry
|September 16, 2020
PubMed
Summary
This summary is machine-generated.

Biotin interference in immunoassays is a growing concern. This study found extremely low biotin levels in UK patient samples, indicating a minimal risk of interference in most laboratory tests.

Keywords:
Biotinimmunoassayinterference

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Area of Science:

  • Clinical Chemistry
  • Laboratory Medicine
  • Biochemistry

Background:

  • Biotin interference in biotin-streptavidin-based immunoassays is increasingly reported.
  • This is due to individuals taking biotin supplements or prescribed biotin.
  • Prevalence data for biotin interference in UK populations were lacking.

Purpose of the Study:

  • To evaluate the prevalence of biotin interference in routine UK serum samples.
  • To assess the risk of biotin interference in laboratory diagnostic tests.

Main Methods:

  • A service evaluation was conducted on 524 anonymized surplus serum samples.
  • Biotin levels were measured in samples where at least one immunoassay test was requested.

Main Results:

  • The median serum biotin level was 0.27 µg/L.
  • All samples had biotin levels below 10 µg/L.
  • 0.8% of samples had biotin levels ≥2.5 µg/L.

Conclusions:

  • The probability of biotin immunoassay interference in the UK patient population is extremely low.
  • Interference is unlikely except for assays with very low interference thresholds, such as the Ortho Troponin I assay (≥2.5 µg/L).