Roseomonas harenae sp. nov., from desert gravel soil

A Gram-stain-negative, aerobic, non-motile, pink-pigmented, coccus bacterium, designated CPCC 101081^T, was isolated from a gravel soil sample collected from Badain Jara desert, PR China. Growth of the isolate occurred at 10-37 °C and pH 5.0-8.0, with optimal growth at 28-32 °C and pH 7.0, respectively. The major cellular fatty acids were summed feature 8 (C_18:1ω7c/C _18:1ω6c), summed feature 3 (C _16:1ω6c/C_16:1ω7c) and C_18:12-OH. Q-10 was detected as the main respiratory quinone. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified phospholipid, an amino-containing lipid and an unidentified glycophospholipid were examined in the polar lipids extraction. The 16S rRNA gene sequence comparison of strain CPCC 101081^T with the available sequences in the GenBank database showed that the isolate was closely related to members of the genus Rosenomonas, with the highest similarity to Roseomonas rosea DSM 14916^T (97.4 %). In the phylogenetic trees based on 16S rRNA gene sequences and the core genomes, strain CPCC 101081^T was included within the clade of the genus Roseomonas, representing a species level, with the closest neighbor of R. rosea DSM 14916^T . The genomic DNA G+C content was 68.7 mol%. The average nucleotide identity and the digital DNA-DNA hybridization values between strain CPCC 101081^T and the related type strains of the genus Roseomonas were all far lower than the cut-off values for definition species. On the basis of above phenotypic and genotypic characteristics, strain CPCC 101081^T is proposed to represent a novel species of the genus Roseomonas with the name Roseomonas harenae sp. nov. strain CPCC 101081^T (=KCTC 62852^T=NBRC 113512^T) is the type strain of the species.