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Updated: Dec 8, 2025
Establishing 3D Endometrial Organoids from the Mouse Uterus
Published on: January 6, 2023
Derivation of endometrial gland organoids from term placenta
Mirna Marinić1, Sarosh Rana2, Vincent J Lynch3
1Department of Human Genetics, The University of Chicago, 920 East 58th Street, CLSC 301, Chicago, IL, 60637, USA; Department of Organismal Biology and Anatomy, The University of Chicago, 1025 East 57th Street, Chicago, IL, 60637, USA.
Abstract:
A limitation of current methods for the generation of endometrial gland organoids is their reliance on decidua isolated from endometrial biopsies or elective abortion. Here we report the establishment of endometrial gland organoids from decidua isolated from term placental membranes. These organoids express typical markers of glandular epithelia such as E-cadherin, Laminin and Cytokeratin 7, and can be propagated in cell culture through multiple passages. Additionally, we identified potential survival factors for the co-culture of organoids and endometrial stromal fibroblasts. These modifications facilitate the generation of patient-specific endometrial gland organoids with known pregnancy outcomes.
