Fieldable flow injection analysis system with chemiluminescence detection capable of quantifying

Area of Science:

• Analytical Chemistry
• Biochemistry
• Biosensor Technology

Background:

• Acetylcholine (ACh) is a crucial neurotransmitter involved in various physiological processes.
• Accurate quantification of ACh is essential for understanding neurological functions and diseases.
• Existing methods for ACh detection may lack sensitivity or require complex procedures.

Purpose of the Study:

• To develop a highly sensitive and accurate biosensor for quantifying acetylcholine (ACh).
• To utilize a flow injection analysis (FIA) system coupled with 1,1"-oxalyldiimidazole chemiluminescence (ODI-CL) detection.
• To validate the performance of the developed biosensor for ACh measurement.

Main Methods:

• A biosensor was designed based on the liquid core waveguide principle.
• Acetylcholine was incubated with a mixture of enzymes (acetylcholinesterase, choline oxidase, horseradish peroxidase) and Amplex Red.
• Chemiluminescence detection using 1,1 -oxalyldiimidazole (ODI-CL) was employed within a flow injection analysis (FIA) system.

Main Results:

• The biosensor demonstrated a linear dynamic range of 0.7–11.3 μM for ACh quantification.
• A high coefficient of determination (R2 = 0.998) indicated excellent linearity.
• The limit of detection (LOD) was as low as 0.14 μM, signifying high sensitivity.
• Recovery tests and linearity studies confirmed the accuracy, precision, and reproducibility of the FIA system with ODI-CL detection.

Conclusions:

• The developed biosensor coupled with FIA and ODI-CL detection provides a sensitive and reliable method for acetylcholine quantification.
• The system exhibits excellent accuracy, precision, and reproducibility, suitable for analytical applications.
• This technology offers a promising tool for research in neurochemistry and related fields.