assisted disposable sensors for quantitative determination of.
Ruchi Dubey Sharma1, Smita Joshi2, Sulbha Amlathe3
1Research Scholar, BUIT, BU, Bhopal, India.
Analytical Methods : Advancing Methods and Applications
|September 17, 2020
Summary
This study presents a new thin-layer chromatography (TLC) sensor for arsenic detection. The sensor uses a colorimetric reaction and scanner analysis, achieving a low detection limit of 0.018 μg mL-1.
Area of Science:
- Analytical Chemistry
- Environmental Science
- Materials Science
Background:
- Arsenic contamination poses significant environmental and health risks.
- Accurate and sensitive detection methods are crucial for monitoring arsenic levels.
- Traditional methods for arsenic determination can be complex and time-consuming.
Purpose of the Study:
- To develop a simple, cost-effective, and sensitive sensor for arsenic determination.
- To utilize thin-layer chromatography (TLC) paper for reagent immobilization.
- To establish a scanner-based detection method for quantitative analysis.
Main Methods:
- Immobilizing a chromogenic reagent (sulfanilic acid and N-(1-naphthyl) ethylene diamine dihydrochloride - NEDA) on TLC paper.
- Reacting immobilized reagents with arsenic to form colored spots (purple to magenta).
- Analyzing color changes using RGB values and MATLAB software for pattern recognition and quantification.
Main Results:
- The sensor demonstrated a linear response in two ranges: 1-10 μg mL⁻¹ and 25-100 μg mL⁻¹.
- A low minimum detection limit of 0.018 μg mL⁻¹ for arsenic was achieved.
- Scanner-based detection of color patterns provided quantitative results.
Conclusions:
- The developed TLC-based sensor offers a sensitive and efficient method for arsenic determination.
- This approach provides a portable and potentially low-cost alternative for arsenic analysis.
- The method shows promise for environmental monitoring and field applications.
