assisted disposable sensors for quantitative determination of
Related Experiment Video
Updated: Oct 16, 2026
Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
Published on: September 24, 2015
Ruchi Dubey Sharma1, Smita Joshi2, Sulbha Amlathe3
1Research Scholar, BUIT, BU, Bhopal, India.
Abstract:
A method for determination of arsenic is described. The sensor was constructed by immobilizing reagents on TLC (thin layer chromatography) paper. The reaction is based on chromogenic reaction of arsenic with a coupling complex of sulfanilic acid and N-(1-naphthyl) ethylene diamine dihydrochloride (NEDA) and formation of purple to magenta spots followed by scanner based detection. Changes in RGB values of color spots on TLC strips create a pattern. The obtained pattern was analyzed using MATLAB software. The proposed sensor is linear in the range 1-10 and 25-100 μg mL -1. The minimum detection limit found was 0.018 μg mL-1of arsenic.
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