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Updated: Dec 8, 2025
Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations
Published on: November 7, 2019
UPLC-ESI-MS analysis of thymosins β4 and β10 in cell lysates: a simple, rapid and sensitive quantification method
David Touboul1, Joanna Wdzieczak-Bakala1, Olivier Laprévote1,2
1Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles, CNRS, Avenue de la Terrasse, 91198, Gif-sur-Yvette Cedex, France. touboul@icsn.cnrs-gif.fr.
Abstract:
Fast and reproducible quantification of thymosins β4 and β10 in different cell cultures was achieved by ultra high performance liquid chromatography coupled to mass spectrometry. We demonstrated that cancer cell lines all exhibit a higher amount of Tβ10 compared to control cells, whereas the level of Tβ4 is drastically depending on cell lines.
