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Updated: Dec 8, 2025
Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
Published on: July 8, 2025
A one-step sensitive dynamic light scattering method for detection using split aptamer fragments.
Xiaohai Yang1, Jiahao Huang1, Qing Wang1
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio- Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha, 410082, China. kmwang@hnu.cn.
A novel one-step method uses dynamic light scattering (DLS) for sensitive and selective detection of adenosine. This breakthrough allows DLS to directly and quantitatively measure small molecules for the first time.
Area of Science:
- Analytical Chemistry
- Biophysical Chemistry
Background:
- Direct detection of small molecules like adenosine is challenging.
- Existing methods may lack sensitivity or selectivity.
- Dynamic Light Scattering (DLS) is typically used for larger particles.
Purpose of the Study:
- To introduce a novel, sensitive, and selective method for adenosine detection.
- To demonstrate the direct and quantitative application of DLS for small molecule analysis.
Main Methods:
- Development of a one-step detection protocol.
- Utilizing dynamic light scattering (DLS) for direct measurement.
- Ensuring high selectivity for adenosine detection.
Main Results:
- Successful direct detection of adenosine was achieved.
- The method demonstrated high sensitivity.
- Quantitative analysis of adenosine using DLS was validated for the first time.
Conclusions:
- Dynamic Light Scattering (DLS) can be effectively applied for direct, sensitive, and quantitative detection of small molecules.
- This represents a significant advancement in analytical methodologies for small molecule quantification.
