A novel biosensor based on sol-gel poly () (PVA)/(titanium dioxide)TiO₂ hybrid material

Area of Science:

• Electrochemistry
• Biosensor Technology
• Materials Science

Background:

• Hydrogen peroxide (H2O2) is a crucial analyte in biological and environmental systems.
• Development of sensitive and selective biosensors is essential for accurate H2O2 detection.
• Hemoglobin (Hb) is a potential enzyme for H2O2 biosensing due to its catalytic activity.

Purpose of the Study:

• To fabricate a novel hydrogen peroxide biosensor.
• To enhance the performance of the biosensor using a hybrid material and nanomaterials.
• To investigate the electrochemical properties and analytical performance of the developed biosensor.

Main Methods:

• Fabrication of a hybrid material by entrapping hemoglobin (Hb) in poly(vinyl alcohol) (PVA)/Titanium dioxide (TiO2).
• Dispersion of multi-walled carbon nanotubes (MWCNTs) into the composite matrix.
• Electrochemical characterization and performance evaluation of the biosensor.

Main Results:

• The hybrid material retained the native biocatalytic activity of Hb.
• MWCNTs enhanced the catalytic performance and electron transfer for H2O2 detection.
• A linear response was observed for H2O2 concentrations from 0.5 to 2.7 μM with a detection limit of 0.01 μM.
• The biosensor demonstrated excellent repeatability, sensitivity, stability, and selectivity.

Conclusions:

• The developed Hb/PVA/TiO2/MWCNT biosensor is a promising platform for sensitive and selective hydrogen peroxide detection.
• The incorporation of MWCNTs significantly improved the biosensor's electrochemical performance.
• The biosensor exhibits favorable analytical characteristics for practical applications.