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High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles
Published on: July 6, 2012
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An ultra sensitive saccharides detection assay using carboxyl functionalized chitosan containing : nanoparticlesprobe
Ashutosh Tiwari1,2, Dohiko Terada1, Prashant K Sharma3
1Biomaterials Center, National Institute for Materials Science, 1-2-1, Sengen, Tsukuba, Ibaraki 305 0047, Japan. tiwari.ashutosh@nims.go.jp ashunpl@gmail.com.
Analytical Methods : Advancing Methods and Applications
|September 17, 2020
Summary
A new electrochemical assay uses a nanocomposite film for sensitive saccharide detection. This novel sensor, featuring amino phenyl boronic acid and europium-doped gadolinium oxide nanoparticles, offers precise analysis for various applications.
Area of Science:
- Electrochemistry
- Nanomaterials Science
- Analytical Chemistry
Background:
- Saccharide detection is crucial for clinical diagnostics, medicine validation, bioscience research, and food analysis.
- Developing sensitive and precise electrochemical assays for saccharides remains a key challenge.
Purpose of the Study:
- To develop a novel saccharide detection assay utilizing a carboxyl functionalized chitosan-based nanocomposite film.
- To immobilize amino phenyl boronic acid (APBA) onto a platinum electrode modified with HOOC-chitosan and Gd2O3:Eu3+ nanoparticles.
Main Methods:
- Covalent immobilization of APBA onto a HOOC-chitosan/Gd2O3:Eu3+ nanocomposite film on a platinum disc electrode.
- Characterization using FT-IR, SEM, contact angle, cyclic voltammetry, XRD, EDX, and TEM.
- Electrochemical detection of saccharides based on pKa changes of boronate esters.
Main Results:
- The developed assay demonstrated excellent electrochemical response to vicinal diols of sugars.
- A wide linear response to boronate esters was observed from 25 nM to 13.5 μM (r2 = 0.963) with good reproducibility.
- The assay showed a good potentiometric response to various saccharides, including glucose.
Conclusions:
- The HOOC-chitosan/Gd2O3:Eu3+ nanocomposite-modified electrode provides a promising platform for precise electrochemical saccharide detection.
- The synergistic effects of the nanocomposite components contribute to the assay's excellent electrochemical activity.
- This assay has potential applications in clinical diagnostics, medicine validation, bioscience research, and food analysis.
