Related Experiment Video
Updated: Dec 8, 2025
07:21
Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
Published on: August 25, 2018
13.2K
Full-length genome sequence of segmented RNA virus from ticks was obtained using small RNA sequencing data
Xiaofeng Xu1, Jinlong Bei2,3, Yibo Xuan1
1Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei, 050024, People's Republic of China.
BMC Genomics
|September 17, 2020
Summary
A new Mogiana tick virus (MGTV) strain was discovered in China using small RNA sequencing. This finding aids in understanding MGTV evolution and the origin of ancient RNA viruses.
Area of Science:
- Virology
- Genomics
- Phylogenetics
Background:
- Mogiana tick virus (MGTV), a Flaviviridae family member, was first identified in Brazil in 2014.
- Related viruses include Jingmen tick virus (JMTV), Kindia tick virus (KITV), and Guangxi tick virus (GXTV).
Purpose of the Study:
- To detect and characterize novel tick-borne viruses using small RNA sequencing.
- To determine the full-length genome sequence of a newly identified MGTV strain.
- To investigate the phylogenetic relationships among MGTV and related viruses.
Main Methods:
- Small RNA sequencing (sRNA-seq) was employed to detect viruses in ticks.
- Full-length genome sequences of the novel MGTV strain were obtained.
- Phylogenetic analysis was conducted to establish evolutionary relationships.
Main Results:
- A new MGTV strain, MGTV-Yunnan2016, was identified in Amblyomma testudinarium ticks in China.
- The complete genome sequences were determined and recommended for NCBI RefSeq inclusion.
- Phylogenetic analysis revealed MGTV, JMTV, KITV, and GXTV form a monophyletic group, potentially linked to ancient supercontinents.
Conclusions:
- This study demonstrates the utility of 5' and 3' sRNAs for generating full-length RNA virus genomes.
- The sRNA-seq method is effective for detecting viruses in small tick pools.
- MGTV offers insights into ancient RNA virus evolution and can serve as a model for phylogeographic studies.
Related Concept Videos
03:21RNA-seq
11.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.4K
01:26Size and Structure of Viral Genomes
501
Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
501
01:29Viruses with RNA Genomes
515
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
515
01:57Sanger Sequencing
770.1K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
770.1K