Quantification of age pigments (lipofuscin)

1. Three methods have hitherto been applied for age pigment quantification: (a) numerically from micrographs; (b) fluorimetrically from histological sections; (c) spectrofluorimetrically from dissolved age pigments. 2. The spectrofluorimetric method is at present the most commonly used technique for quantification of age pigments. 3. By comparing the related publications since introduction of the spectrofluorimetric method, it has become apparent that few authors specify fluorimetrically significant factors, such as temperature and pH which influence the sample fluorescence during measurement. 4. Recent developments in fluorimetrical age pigment quantification using chloroform/methanol as solvent have additionally revealed the necessity to measure and present the age pigments dissolved in both phases: the polar and non-polar solvents.