Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Anatomy of the Intestines01:23

Anatomy of the Intestines

65.4K
Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occurs in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
65.4K
Gastrulation01:56

Gastrulation

53.1K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
53.1K
Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

2.7K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Factors associated with acute radiation-induced nocturia in localized prostate cancer treated with proton beam therapy.

Journal of radiation research·2026
Same author

New developments and applications of human organoids.

Nature reviews. Molecular cell biology·2026
Same author

Complementary volume electron microscopy-based approaches reveal ultrastructural changes in germline intercellular bridges.

Journal of cell science·2026
Same author

A stereotyped glial attachment determines the morphology and function of neuronal cilia.

bioRxiv : the preprint server for biology·2026
Same author

Interferon-γ selectively promotes survival of alveolar progenitor cells in a human lung organoid model.

The EMBO journal·2026
Same author

Discovery of a secreted <i>Bacteroides fragilis</i> mucinase that cleaves mucins with bis-T O-glycans through a carbohydrate binding module-dependent mechanism.

Gut microbes·2026

Related Experiment Video

Updated: May 6, 2026

Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality
10:56

Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality

Published on: May 5, 2022

14.5K

Homeostatic mini-intestines through scaffold-guided organoid morphogenesis.

Mikhail Nikolaev1, Olga Mitrofanova1, Nicolas Broguiere1

  • 1Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences (SV), École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.

Nature
|September 17, 2020
PubMed
Summary

Researchers engineered perfusable mini-gut tubes from intestinal stem cells. This breakthrough overcomes organoid limitations, enabling longer lifespan, microbial colonization, and better disease modeling.

More Related Videos

Author Spotlight: Generation and Manipulation of Rat Intestinal Organoids
09:49

Author Spotlight: Generation and Manipulation of Rat Intestinal Organoids

Published on: June 23, 2023

5.1K
Innervation of Human Intestinal Organoids
07:23

Innervation of Human Intestinal Organoids

Published on: January 17, 2025

933

Related Experiment Videos

Last Updated: May 6, 2026

Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality
10:56

Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality

Published on: May 5, 2022

14.5K
Author Spotlight: Generation and Manipulation of Rat Intestinal Organoids
09:49

Author Spotlight: Generation and Manipulation of Rat Intestinal Organoids

Published on: June 23, 2023

5.1K
Innervation of Human Intestinal Organoids
07:23

Innervation of Human Intestinal Organoids

Published on: January 17, 2025

933

Area of Science:

  • Tissue engineering
  • Stem cell biology
  • Organoid technology

Background:

  • Current epithelial organoids, derived from stem cells, have limitations due to their cystic architecture, restricting lifespan, size, and experimental manipulation.
  • Existing organoid models hinder homeostasis and in-depth study of tissue and disease biology.

Purpose of the Study:

  • To engineer novel, functional intestinal organoids with improved architecture and physiological relevance.
  • To overcome the limitations of conventional organoids for advanced tissue and disease modeling.

Main Methods:

  • Utilizing tissue engineering principles and intrinsic cell self-organization to guide intestinal stem cells.
  • Developing tube-shaped epithelial structures with accessible lumens and in vivo-like spatial organization.
  • Connecting engineered mini-gut tubes to a perfusion system for continuous cell removal and microbial colonization.

Main Results:

  • Successfully generated tube-shaped intestinal epithelia with accessible lumens and crypt-villus-like domains.
  • Perfusion enabled prolonged tissue lifespan by removing dead cells and allowed for microbial colonization.
  • The engineered mini-guts exhibited key physiological hallmarks, included rare cell types, and demonstrated regenerative capacity.

Conclusions:

  • The developed mini-gut tubes represent a significant advancement over conventional organoids.
  • This organoid-on-a-chip approach offers a more physiologically relevant platform for studying intestinal biology and diseases.
  • The technique is broadly applicable for creating functional organoids with enhanced shapes, sizes, and functions.