Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

DNA strand exchanges.

J D Griffith¹, L D Harris

¹Lineberger Cancer Research Center, University of North Carolina, Chapel Hill.

CRC Critical Reviews in Biochemistry

|January 1, 1988

Summary

This summary is machine-generated.

RecA and UvsX proteins catalyze DNA strand exchange through three steps: joining, exchange, and release. This review details the structure of intermediates and the role of paranemic joints in DNA repair and recombination.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Loss of mucosal CD103+ DCs and IL-17+ and IL-22+ lymphocytes is associated with mucosal damage in SIV infection.

Mucosal immunology·2012

Same author

The effects of children on divorce and re-marriage: a multivariate analysis of life table probabilities.

Population studies·2011

Same author

The 1989 annual meeting.

Conservation biology : the journal of the Society for Conservation Biology·2010

Same author

Compromised gastrointestinal integrity in pigtail macaques is associated with increased microbial translocation, immune activation, and IL-17 production in the absence of SIV infection.

Mucosal immunology·2010

Same author

Innocuous Transportation of the Dead.

Public health papers and reports·2009

Same author

Validation of NMR relaxation exchange time measurements in porous media.

The Journal of chemical physics·2007

Same journal

Methionine biosynthesis in Enterobacteriaceae: biochemical, regulatory, and evolutionary aspects.

CRC critical reviews in biochemistry·1988

Same journal

Viral RNA polymerases.

CRC critical reviews in biochemistry·1988

Same journal

Positional isotope exchange.

CRC critical reviews in biochemistry·1988

Same journal

Glutathione transferases--structure and catalytic activity.

CRC critical reviews in biochemistry·1988

Same journal

Structure-stability relationship in proteins: fundamental tasks and strategy for the development of stabilized enzyme catalysts for biotechnology.

CRC critical reviews in biochemistry·1988

Same journal

Interpreting the behavior of enzymes: purpose or pedigree?

CRC critical reviews in biochemistry·1988

See all related articles

Area of Science:

Molecular Biology
Biochemistry
Structural Biology

Background:

DNA strand exchange is a fundamental process in DNA repair and recombination.
RecA protein (E. coli) and UvsX protein (T4 phage) are key enzymes catalyzing this reaction.

Purpose of the Study:

To elucidate the three-step mechanism of RecA/UvsX-mediated DNA strand exchange.
To describe the structure of reaction intermediates and the role of paranemic joints.

Main Methods:

Biochemical assays to study strand exchange reactions.
Electron microscopy to visualize protein-DNA complexes and intermediates.
In vitro DNA pairing experiments with various DNA substrates.

Main Results:

Related Experiment Videos

DNA strand exchange proceeds via three distinct steps: joining, envelopment/exchange, and product release.

Paranemic joints are identified as a major pathway in DNA molecule joining.

The structure of intermediates provides insights into the reaction mechanism.

Conclusions:

RecA and UvsX proteins utilize a conserved three-step mechanism for DNA strand exchange.
Paranemic joint formation is crucial for efficient DNA joining and may have in vivo significance.
Understanding these mechanisms is vital for comprehending DNA repair and recombination pathways.