Related Experiment Video
Updated: Dec 8, 2025
Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
Published on: April 21, 2023
The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles
Prashik Parvekar1, Jayant Palaskar2, Sandeep Metgud3
1Pacific Academy of Higher Education, Research University, Udaipur, India.
Aim:
To determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against Staphylococcus aureus (S. aureus).Methodology: The antimicrobial efficacy of the silver nanoparticles was determined by the standard methods of Clinical and Laboratory Standards Institute (CLSI). Different concentrations of silver nanoparticles were prepared, and MIC was calculated by tube macro-dilution method. The MBC was determined by the lowest concentration that kills 99.9% of the initial bacterial population. The data were analyzed by ANOVA and Tukey's post hoc test using SPSS software.
Results:
The MIC and MBC of silver nanoparticles against S. aureus was found to be 0.625 mg/ml.
Conclusion:
The result obtained from this study shows that silver nanoparticles have potential bactericidal effects against S. aureus at a concentration of 0.625 mg/ml. Silver nanoparticles can be incorporated in the root canal medicaments, sealers and irrigants as it possess potent antimicrobial efficacy against S. aureus.
Insights
Silver nanoparticles show strong bactericidal effects against Staphylococcus aureus (S. aureus). This study found the minimum inhibitory and bactericidal concentrations to be 0.625 mg/ml, suggesting potential dental applications.
Area of Science:
- Nanotechnology
- Microbiology
- Biomaterials
Background:
- Staphylococcus aureus (S. aureus) is a common pathogen.
- Antimicrobial resistance necessitates novel therapeutic agents.
- Silver nanoparticles (AgNPs) exhibit broad-spectrum antimicrobial activity.
Purpose of the Study:
- To quantify the antimicrobial efficacy of silver nanoparticles against S. aureus.
- To determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of AgNPs.
Main Methods:
- Antimicrobial susceptibility testing followed Clinical and Laboratory Standards Institute (CLSI) guidelines.
- MIC determined via tube macro-dilution method.
- MBC determined as the lowest concentration killing 99.9% of S. aureus.
Main Results:
- The MIC of silver nanoparticles against S. aureus was 0.625 mg/ml.
- The MBC of silver nanoparticles against S. aureus was also 0.625 mg/ml.
Conclusions:
- Silver nanoparticles demonstrate significant bactericidal potential against S. aureus at 0.625 mg/ml.
- AgNPs show promise for incorporation into dental materials like root canal medicaments, sealers, and irrigants due to their potent antimicrobial properties.
More Related Videos
11:19Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties
Published on: May 10, 2018
07:40Production of Metal Nanoparticles by Pulsed Laser-ablation in Liquids: A Tool for Studying the Antibacterial Properties of Nanoparticles
Published on: June 2, 2017
