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Updated: Dec 8, 2025

Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
Molecular Tension Microscopy of E-Cadherin During Epithelial-Mesenchymal Transition
Helena Canever1, Pietro Salvatore Carollo1, Romain Fleurisson1
1Institut Jacques Monod, Université de Paris, CNRS, Paris, France.
Abstract:
Molecular Tension Microscopy has been increasingly used in the last years to investigate mechanical forces acting in cells at the molecular scale. Here, we describe a protocol to image the tension of the junctional protein E-cadherin in cultured epithelial cells undergoing Epithelial-Mesenchymal Transition (EMT). We report how to prepare cells and induce EMT, and how to acquire, analyze, and quantitatively interpret FRET data.
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