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Updated: Dec 8, 2025
A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
Published on: March 17, 2023
Activity-Based NIR Enzyme Fluorescent Probes for the Diagnosis of Tumors and Image-Guided Surgery
Haidong Li1, Dayeh Kim1, Qichao Yao2
1Department of Chemistry and Nanoscience, Ewha Womans University, Seoul, 03760, Korea.
Abstract:
Near-infrared (NIR) activatable fluorescent probes have been considered to be the effective edge tools for the investigation of cell biology and disease diagnosis because of their outstanding advantages. Related genes involved in tumor genesis and progression regulate the overexpression of certain enzymes. Owing to the distinctive characteristics of quick reaction time and favorable pharmacokinetics, enzyme-reactive NIR optical probes have shown great potential in the diagnosis of tumorigenesis and in image-guided intraoperative surgeries with high signal-to-noise ratios. In this review, we mainly summarize the latest advancements in enzyme-reactive NIR fluorescent probes from design strategy to biomedical application. Specifically, some challenges and prospects in this field are presented at the end of the review, which will be beneficial to innovatively construct new multifunctional fluorescent probes and actively promote their clinical transformation in the future.
Insights
Enzyme-reactive near-infrared (NIR) fluorescent probes offer advanced tools for cell biology and disease diagnosis. These probes show great potential for tumor diagnosis and image-guided surgery due to their rapid response and favorable pharmacokinetics.
Area of Science:
- Biomedical Engineering
- Molecular Imaging
- Optical Probes
Background:
- Near-infrared (NIR) activatable fluorescent probes are valuable tools in cell biology and disease diagnosis.
- Enzyme activity is often altered in diseases like cancer, making enzymes targets for diagnostic probes.
- NIR probes offer advantages like deep tissue penetration and high signal-to-noise ratios.
Purpose of the Study:
- To review recent advancements in enzyme-reactive NIR fluorescent probes.
- To discuss design strategies and biomedical applications of these probes.
- To identify challenges and future prospects for developing novel probes.
Main Methods:
- Literature review of enzyme-reactive NIR fluorescent probes.
- Analysis of probe design principles and their correlation with enzyme activity.
- Evaluation of probe performance in biomedical applications, including disease diagnosis and surgery.
Main Results:
- Enzyme-reactive NIR probes demonstrate significant potential for early tumor detection and image-guided surgery.
- Specific enzyme-reactive probes exhibit high sensitivity and selectivity for disease biomarkers.
- Recent progress includes innovative probe designs enhancing reaction speed and pharmacokinetic profiles.
Conclusions:
- Enzyme-reactive NIR fluorescent probes are crucial for advancing biomedical imaging and diagnostics.
- Further development is needed to overcome current challenges and facilitate clinical translation.
- Future research should focus on creating multifunctional probes for improved diagnostic and therapeutic outcomes.
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