Streptomyces arboris sp. nov., isolated from Populus euphratica wetland soil

A novel actinobacterial strain (TRM 68085^T) was isolated from soil of Populus euphratica wetland. A polyphasic approach was used to study the taxonomy of TRM 68085^T and the results showed a range of phylogenetic and chemotaxonomic properties consistent with those of the genus Streptomyces. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain TRM 68085^T showed the highest similarity value to Streptomyces capitiformicae 1H-SSA4^T (98.6 %), and phylogenetically clustered with Streptomyces kanasensis ZX01^T(97.5 %) and Streptomyces ipomoeae NBRC 13050^T (97.4 %). The genomic DNA G+C content of strain TRM 68085^T based on the genome sequence was 71.4 mol%. The levels of DNA-DNA relatedness between the genome of the isolate and its nearest phylogenetic neighbours, S. capitiformicae 1H-SSA4^T, S. kanasensis ZX01^T and S. ipomoeae NBRC 13050^T, were 19.2±0.4, 21.8±0.5 and 19.3±0.6 %, respectively. Chemotaxonomic data revealed that strain TRM 68085^T possessed MK-9(H₆) and MK-9(H₈) as the predominant menaquinones. ll-Diaminopimelic acid and a small amount of meso-diaminopimelic acid were the diagnostic diamino acids. Ribose, xylose, glucose and galactose were the whole-cell sugars. The major cellular fatty acids were C_16 : 0 (25.4 %) and iso-C_16 : 0 (18.3 %). On the basis of these genotypic and phenotypic data, it is concluded that strain TRM 68085^T represents a novel species of the genus Streptomyces, for which the name Streptomyces arboris sp. nov. is proposed. The type strain is TRM 68085^T (=CCTCC AA2019031^T=LMG 31492^T).