Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genomic mapping by fingerprinting random clones: a mathematical analysis.

E S Lander1, M S Waterman

  • 1Whitehead Institute for Biomedical Research, Cambridge Center, Massachusetts 02142.

Genomics
|April 1, 1988
PubMed
Summary

This study presents formulas to evaluate how fingerprinting schemes impact physical mapping project progress. Choosing the right fingerprinting method is crucial for efficient genome mapping and assembly.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rare germline variants in ATM are associated with chronic lymphocytic leukemia.

Leukemia·2017
Same author

Whole-genome sequencing reveals activation-induced cytidine deaminase signatures during indolent chronic lymphocytic leukaemia evolution.

Nature communications·2015
Same author

Topological classification and enumeration of RNA structures by genus.

Journal of mathematical biology·2012
Same author

SNP genotyping defines complex gene-flow boundaries among African malaria vector mosquitoes.

Science (New York, N.Y.)·2010
Same author

Genome sequence, comparative analysis, and population genetics of the domestic horse.

Science (New York, N.Y.)·2009
Same author

cDNA sequences for transcription factors and signaling proteins of the hemichordate Saccoglossus kowalevskii: efficacy of the expressed sequence tag (EST) approach for evolutionary and developmental studies of a new organism.

The Biological bulletin·2008

Area of Science:

  • Genomics
  • Bioinformatics
  • Computational Biology

Background:

  • Physical mapping projects are essential for genome sequencing and assembly.
  • Recent projects for Escherichia coli, Saccharomyces cerevisiae, and Caenorhabditis elegans highlight the need for efficient mapping strategies.
  • Current physical mapping relies on fingerprinting clones from recombinant libraries to infer overlaps.

Purpose of the Study:

  • To derive formulas that quantify the impact of different fingerprinting schemes on physical mapping project progress.
  • To provide analytic considerations for selecting optimal fingerprinting strategies.
  • To guide the design of future genome physical mapping initiatives.

Main Methods:

  • Development of mathematical formulas to model the relationship between fingerprinting characteristics and mapping efficiency.

Related Experiment Videos

  • Analysis of how variations in fingerprinting schemes affect clone overlap detection.
  • Evaluation of analytic considerations for choosing appropriate fingerprinting methods.

    • Main Results:

    • Formulas derived demonstrate a direct correlation between fingerprinting scheme properties and project progress.
    • The choice of fingerprint and overlap rules significantly influences the speed and accuracy of physical map assembly.
    • Analytic insights are provided for optimizing fingerprinting strategies.

    Conclusions:

    • The selection of a fingerprinting scheme is a critical factor in the success and efficiency of physical mapping projects.
    • Understanding the analytic implications of fingerprinting choices can lead to improved genome mapping methodologies.
    • This work offers a framework for rationally designing fingerprinting strategies for various genomic organisms.