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Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering
Published on: March 1, 2016
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Multifunctional-imprinted nanocomposite membranes with thermo-responsive biocompatibility for selective/controllable
Ming Yan1, Yilin Wu2, Kaicheng Zhang3
1Institute of Green Chemistry and Chemical Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Journal of Colloid and Interface Science
|September 17, 2020
Summary
This study developed thermo-responsive molecularly imprinted nanocomposite membranes (MINCMs) for ovalbumin (Ova) separation. The MINCMs demonstrated excellent Ova rebinding capacity and controllable cell adhesion, showing potential for biomolecule separation and tissue engineering applications.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Separation Science
Background:
- Dopamine polymerization offers biomimetic modification strategies for advanced materials.
- Thermo-responsive polymers enable dynamic control over material properties.
- Molecular imprinting creates specific recognition sites for target molecules.
Purpose of the Study:
- To synthesize molecularly imprinted nanocomposite membranes (MINCMs) with thermo-responsive properties for ovalbumin (Ova) separation.
- To evaluate the rebinding capacity, selectivity, and stability of the developed MINCMs.
- To assess the biocompatibility and cell adhesion control of the Ova-bound MINCMs.
Main Methods:
- Au/SiO2 multilevel structures were fabricated on polydopamine (pDA) modified membranes.
- Photoinitiated atom transfer radical polymerization (pATRP) was used to create Ova-imprinted sites.
- Thermo-responsive poly(N-isopropylacrylamide) was incorporated into the imprinted sites.
- Rebinding capacity, selectivity, and cell adhesion studies were performed at different temperatures.
Main Results:
- MINCMs exhibited excellent Ova rebinding capacity (33.26 mg/g) and selectivity factor (3.06) at 37 °C.
- The Ova-bound MINCMs demonstrated excellent biocompatibility, with cell adhesion and viability comparable to bare glass.
- Efficient and rapid regulation of cell adhesion/detachment was achieved over 10 temperature-switch cycles.
Conclusions:
- The developed MINCMs offer a promising platform for thermo-responsive biomolecule separation.
- The material exhibits excellent biocompatibility and controllable cell adhesion, suitable for tissue engineering.
- The MINCMs show robust performance under continuous operation, indicating potential for practical applications.
