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Related Experiment Video

Updated: Dec 8, 2025

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

Engineering lytic polysaccharide monooxygenases (LPMOs).

Zarah Forsberg¹, Anton A Stepnov¹, Guro Kruge Nærdal²

¹Faculty of Chemistry, Biotechnology and Food Science, NMBU-Norwegian University of Life Sciences, Ås, Norway.

Methods in Enzymology

|September 18, 2020

Summary

Enzyme engineering of lytic polysaccharide monooxygenases (LPMOs) aims to create robust biocatalysts for industrial applications. Challenges include assessing LPMO functionality due to side reactions complicating screening methods.

Keywords:

Cellulose Chitin Co-evolution Enzyme engineering Hydrogen peroxide Lytic polysaccharide monooxygenase Oxidative damage Regioselectivity Substrate specificity Thermostability

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OLIgo Mass Profiling OLIMP of Extracellular Polysaccharides

OLIgo Mass Profiling OLIMP of Extracellular Polysaccharides

Published on: June 20, 2010

Area of Science:

Biochemistry
Enzymology
Biotechnology

Background:

Lytic polysaccharide monooxygenases (LPMOs) are copper-containing enzymes that hydroxylate glycosidic bonds in recalcitrant polysaccharides.
Their discovery in 2010 sparked interest due to their oxidative power and synergy with hydrolytic enzymes.

Purpose of the Study:

To review LPMO engineering efforts for industrial biocatalyst development.
To highlight challenges in assessing LPMO functionality and reaction mechanisms.

Main Methods:

Literature review of LPMO engineering strategies.
Discussion of difficulties in LPMO activity assays and screening.

Main Results:

LPMO engineering targets include enhanced robustness, activity, specificity, and novel functions.
Assessing LPMO functionality is complicated by side reactions and requires improved methodologies.

Conclusions:

Robust LPMOs are needed for industrial saccharification, but engineering requires better understanding of active sites and mechanisms.
Development of reliable screening methods is crucial for advancing LPMO engineering.