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Author Spotlight: Advanced Techniques for Characterizing Tissue Mineralization in Bone Regeneration Research
Published on: September 27, 2024
Automated Raman Spectral Preprocessing of Bone and Other Musculoskeletal Tissues
Francis W L Esmonde-White1, Matthew V Schulmerich1, Karen A Esmonde-White2
1Dept. of Chemistry, Univ. of Michigan, 930 N. University, Ann Arbor, MI, USA 48109-1055.
Abstract:
Raman spectroscopy of bone is complicated by fluorescence background and spectral contributions from other tissues. Full utilization of Raman spectroscopy in bone studies requires rapid and accurate calibration and preprocessing methods. We have taken a step-wise approach to optimize and automate calibrations, preprocessing and background correction. Improvements to manual spike removal, white light correction, software image rotation and slit image curvature correction are described. Our approach is concisely described with a minimum of mathematical detail.
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