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LncRNA JPX promotes cervical cancer progression by modulating miR-25-3p/SOX4 axis

Xia Chen1, Jingxiu Yang1, Yuping Wang1

  • 1Department of Gynaecology and Obstetrics, The Affiliated Lianyungang Oriental Hospital of Xuzhou Medical University, No. 57 Zhong'hua Rest Road, Lianyun District, Lianyungang, 222042 Jiangsu China.

Cancer Cell International
|September 18, 2020
PubMed
Abstract

Insights

The long noncoding RNA JPX promotes cervical cancer by regulating miR-25-3p and SOX4. This finding suggests JPX as a potential therapeutic target for cervical cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are implicated in tumor development.
  • The role of lncRNA JPX in cervical cancer (CC) is currently unknown.
  • JPX acts as a molecular switch for X-chromosome inactivation.

Purpose of the Study:

  • To investigate the functional role and mechanism of lncRNA JPX in cervical cancer.
  • To explore the relationship between JPX, miR-25-3p, and SOX4 in CC.
  • To determine if the JPX/miR-25-3p/SOX4 axis is a potential therapeutic target for CC.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and western blotting assessed JPX, miR-25-3p, and SOX4 expression.
  • Bioinformatic analysis (StarBase v2.0), luciferase reporter, and RNA immunoprecipitation assays investigated the JPX-miR-25-3p interaction.
  • Cell proliferation, migration, and invasion were evaluated using EdU, CCK-8, and Transwell assays.
  • In vivo tumor xenograft assays in nude mice demonstrated the axis's role in CC progression.

Main Results:

  • JPX was significantly upregulated, while miR-25-3p was downregulated in CC tissues and cell lines.
  • JPX overexpression enhanced, and knockdown inhibited, HeLa cell proliferation, migration, and invasion.
  • JPX knockdown suppressed HeLa cell viability and tumor growth by upregulating miR-25-3p and downregulating SOX4.
  • JPX expression negatively correlated with miR-25-3p in CC tissues.

Conclusions:

  • lncRNA JPX promotes cervical cancer progression by modulating the miR-25-3p/SOX4 axis.
  • The JPX/miR-25-3p/SOX4 pathway represents a potential therapeutic target for cervical cancer.
  • Understanding lncRNA JPX's mechanism offers insights into cervical cancer development.
Keywords:
Cervical cancerJPXSOX4miR-25-3p

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