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Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins

Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins

Published on: November 17, 2016

An improved pollen number counting method using a cell counter and mesh columns.

Hiroyuki Kakui¹, Eriko Tsurisaki², Hidenori Sassa³

¹Graduate School of Science and Technology, Niigata University, Niigata, Niigata, 950-2181 Japan.

|September 18, 2020

Summary

A new method accurately counts Japanese cedar (Cryptomeria japonica) pollen grains. This technique is crucial for understanding pollinosis and can be adapted for other plant species.

Keywords:

CASY cell counter Japanese cedar (Cryptomeria japonica)Pollen number Pollen size

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Area of Science:

Botany
Plant Science
Allergology

Background:

Pollen quantification is vital for evolutionary, agricultural, and medical research.
Cupressaceae family trees, particularly Japanese cedar (Cryptomeria japonica), are significant global causes of pollinosis.
Existing pollen counting methods are unsuitable for Japanese cedar due to its strobilus structure.

Purpose of the Study:

To develop an improved and accurate method for counting Japanese cedar pollen grains.
To address the limitations of current pollen enumeration techniques for specific plant species.

Main Methods:

A precise cell counter was combined with custom-made mesh columns (100- and 20-μm).
Male strobili were gently crushed, and debris was efficiently removed using the mesh columns.
The method was validated by detecting differences in pollen size and number between two Japanese cedar clones.

Main Results:

Successfully quantified pollen from Japanese cedar (Cryptomeria japonica) using the novel method.
Demonstrated variability in pollen production between different Japanese cedar clones.
The method proved effective in removing debris and counting pollen.

Conclusions:

The developed method is effective for Japanese cedar and applicable to other Cupressaceae species.
This technique can be adapted for a wider range of plant species, including wheat, without requiring anthesis.
Efficient debris removal and precise pollen counting are key advantages of this improved method.