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Related Experiment Videos

Unexpected divergence and molecular coevolution in yeast plasmids.

J A Murray1, G Cesareni, P Argos

  • 1European Molecular Biology Laboratory, Heidelberg Federal Republic of Germany.

Journal of Molecular Biology
|April 5, 1988
PubMed
Summary

Yeast nuclear plasmids share a common ancestor despite lacking DNA homology. Functional protein interactions were conserved even with significant DNA sequence divergence, suggesting molecular drive in plasmid evolution.

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Area of Science:

  • Molecular biology
  • Yeast genetics
  • Evolutionary biology

Background:

  • Four related yeast species harbor multicopy nuclear plasmids.
  • These plasmids share a common ancestor, indicated by molecular architecture, yet lack DNA sequence homology.

Purpose of the Study:

  • Investigate the evolutionary relationships and functional conservation of yeast nuclear plasmids.
  • Determine the mechanisms maintaining plasmid function despite DNA sequence divergence.

Main Methods:

  • Comparative sequence analysis of plasmid-encoded proteins.
  • Phylogenetic analysis using sequence alignment data.
  • Functional assessment of protein-plasmid interactions.

Main Results:

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  • Plasmids encode three essential maintenance proteins with varying conservation levels.
  • Two protein groups were alignable; the third showed extreme divergence, preventing alignment.
  • Functional protein-plasmid interactions were conserved despite lack of amino acid sequence conservation.
  • Phylogenetic analyses yielded inconsistent results, contradicting taxonomic relationships.

    • Conclusions:

    • Conserved functional interactions, despite DNA divergence, suggest molecular drive in yeast plasmid evolution.
    • Inconsistent phylogenetic signals highlight complex evolutionary dynamics.
    • Extreme sequence divergence in some proteins challenges traditional evolutionary interpretations.