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On the lineage of Aspergillus fumigatus isolates in common laboratory use
Margherita Bertuzzi1,2, Norman van Rhijn1,2, Sven Krappmann3
1Manchester Fungal Infection Group, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Core Technology Facility, Manchester, UK.
Abstract:
The origin of isolates routinely used by the community of Aspergillus fumigatus researchers is periodically a matter of intense discussion at our centre, as the construction of recombinant isolates have sometimes followed convoluted routes, the documentation describing their lineages is fragmented, and the nomenclature is confusing. As an aide memoir, not least for our own benefit, we submit the following account and tabulated list of strains (Table 1) in an effort to collate all of the relevant information in a single, easily accessible document. To maximise the accuracy of this record we have consulted widely amongst the community of Medical Mycologists using these strains. All the strains described are currently available from one of these organisations, namely the Fungal Genetics Stock Centre (FGSC), FungiDB, Ensembl Fungi and The National Collection of Pathogenic Fungi (NCPF) at Public Health England. Display items from this manuscript are also featured on FungiDB.
Lay Abstract:
We present a concise overview on the definition, origin and unique genetic makeup of the Aspergillus fumigatus isolates routinely in use by the fungal research community, to aid researchers to describe past and new strains and the experimental differences observed more accurately.
Insights
This study clarifies the origins and genetic makeup of commonly used Aspergillus fumigatus research strains. It provides a vital resource for accurate strain description and experimental reproducibility in fungal research.
Area of Science:
- Medical Mycology
- Fungal Genetics
- Microbiology
Background:
- The routine use of Aspergillus fumigatus isolates in research is complicated by unclear origins and fragmented lineage documentation.
- Confusing nomenclature and complex construction histories of recombinant strains hinder accurate record-keeping.
Purpose of the Study:
- To provide a clear overview of the definition, origin, and genetic makeup of Aspergillus fumigatus isolates used in research.
- To aid researchers in accurately describing past and new strains and observed experimental differences.
Main Methods:
- Consultation with the Medical Mycology community to maximize record accuracy.
- Collation of information on strain lineages and documentation.
- Tabulation of strain information for accessibility.
Main Results:
- A comprehensive account and tabulated list of Aspergillus fumigatus strains is presented.
- Information on strain availability from key organizations (FGSC, FungiDB, Ensembl Fungi, NCPF) is provided.
- Display items are featured on FungiDB.
Conclusions:
- This work serves as an aide-mémoire for researchers, consolidating essential information on Aspergillus fumigatus strains.
- Improved clarity on strain origins and genetics promotes more accurate research and reporting.
- Accessible documentation facilitates better understanding and utilization of Aspergillus fumigatus research strains.
