Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Electron Microscopy01:25

Overview of Electron Microscopy

12.5K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
12.5K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

4.9K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
4.9K
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

5.0K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
5.0K
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

6.6K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
6.6K
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

6.4K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
6.4K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.7K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PI-FPM: pupil initialization for Fourier ptychographic microscopy directly from measurement data.

Optics express·2026
Same author

The pleuroparenchymal fibroelastosis atlas reveals aberrant cell states and their zonation as an alternate roadmap to lung fibrosis.

Science advances·2026
Same author

Ultrastructural analysis of bovine trophoblast giant cells during their migration by serial block-face scanning electron microscopy (SBF-SEM).

Placenta·2026
Same author

RHOT Proteins Link Mitochondrial Motility to Cardiomyocyte Sarcomere Maturation.

Circulation research·2026
Same author

Targeting fused in sarcoma (FUS): a novel antisense strategy for treating idiopathic pulmonary fibrosis.

Signal transduction and targeted therapy·2026
Same author

Extracellular BRICK1 drives heart repair after myocardial infarction in mice.

Science translational medicine·2026
Same journal

May in focus in HCB: organelles-mitochondria and peroxisomes.

Histochemistry and cell biology·2026
Same journal

Altered expression of Toll-like receptor 9 in the lung tissue of adult mice generated by in vitro embryo culture and embryo transfer.

Histochemistry and cell biology·2026
Same journal

Dynamic changes in OTULIN and progranulin levels in experimental myocardial infarction and cardiac remodeling.

Histochemistry and cell biology·2026
Same journal

Eosinophil-associated matrix remodeling in a sterile granulomatous inflammation model: a temporal histopathological analysis.

Histochemistry and cell biology·2026
Same journal

Cellular accumulation of lipofuscin in the heart: implications in health and disease.

Histochemistry and cell biology·2026
Same journal

From lipofuscin accumulation to cellular dysfunction: a focus on liver pathophysiology.

Histochemistry and cell biology·2026
See all related articles

Related Experiment Video

Updated: Dec 8, 2025

Author Spotlight: Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections
07:17

Author Spotlight: Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections

Published on: October 6, 2023

7.2K

Volume electron microscopy: analyzing the lung.

Jan Philipp Schneider1,2, Jan Hegermann3,4,5, Christoph Wrede1,2,6

  • 1Institute of Functional and Applied Anatomy, Hannover Medical School, 30625, Hannover, Germany.

Histochemistry and Cell Biology
|September 18, 2020
PubMed
Summary
This summary is machine-generated.

Electron microscopy offers lung researchers detailed 3D ultrastructure insights. This review details various electron microscopy techniques and their applications in lung research, including data processing for 3D modeling.

Keywords:
3D reconstructionArray tomographyElectron tomographyFocused ion beam scanning electron microscopyLungSerial block-face scanning electron microscopySerial sectioning transmission electron microscopyVolume electron microscopy

More Related Videos

Author Spotlight: Advances in Quantifying Microvascular Density in Aging Murine Lungs
10:00

Author Spotlight: Advances in Quantifying Microvascular Density in Aging Murine Lungs

Published on: January 3, 2025

3.7K
A Standardized Method for Measuring Internal Lung Surface Area via Mouse Pneumonectomy and Prosthesis Implantation
08:46

A Standardized Method for Measuring Internal Lung Surface Area via Mouse Pneumonectomy and Prosthesis Implantation

Published on: July 26, 2017

13.7K

Related Experiment Videos

Last Updated: Dec 8, 2025

Author Spotlight: Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections
07:17

Author Spotlight: Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections

Published on: October 6, 2023

7.2K
Author Spotlight: Advances in Quantifying Microvascular Density in Aging Murine Lungs
10:00

Author Spotlight: Advances in Quantifying Microvascular Density in Aging Murine Lungs

Published on: January 3, 2025

3.7K
A Standardized Method for Measuring Internal Lung Surface Area via Mouse Pneumonectomy and Prosthesis Implantation
08:46

A Standardized Method for Measuring Internal Lung Surface Area via Mouse Pneumonectomy and Prosthesis Implantation

Published on: July 26, 2017

13.7K

Area of Science:

  • Biomedical research
  • Pulmonology
  • Microscopy

Background:

  • Electron microscopy (EM) has been crucial in lung research since the mid-20th century.
  • EM confirmed the continuous alveolar epithelium and the surfactant lining layer.
  • Early EM techniques provided foundational insights into lung ultrastructure.

Purpose of the Study:

  • To provide an overview of advanced electron microscopy techniques for lung research.
  • To discuss the application, implementation, and data processing for 3D modeling in lung ultrastructure studies.
  • To highlight how different EM techniques address specific research questions based on volume and resolution.

Main Methods:

  • Serial sectioning transmission electron microscopy (TEM)
  • Electron tomography
  • Serial block-face scanning electron microscopy (SBF-SEM)
  • Focused ion beam scanning electron microscopy (FIB-SEM)
  • Array tomography

Main Results:

  • These techniques enable the investigation of lung ultrastructure in three dimensions.
  • Each technique offers distinct resolutions and volume coverage, suiting diverse research needs.
  • The review details practical aspects and data processing for generating 3D models from EM data.

Conclusions:

  • Advanced electron microscopy techniques have revolutionized the study of lung ultrastructure.
  • The choice of technique depends on the specific research question and desired resolution/volume.
  • Understanding data processing is key to generating meaningful 3D models of lung tissue.