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Updated: Dec 8, 2025
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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
Published on: September 18, 2020
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Profiling DNA Methylation Genome-Wide in Single Cells.
António Galvão1,2,3, Gavin Kelsey4,5
1Institute of Animal Reproduction and Food Research of PAS, Olsztyn, Poland.
Methods in Molecular Biology (Clifton, N.J.)
|September 18, 2020
Summary
Single-cell bisulfite sequencing (scBS-seq) provides detailed DNA methylation profiles. This protocol in a 96-well plate format is suitable for studying methylation in rare cells like oocytes and early embryos.
Area of Science:
- Epigenetics
- Genomics
- Molecular Biology
Background:
- DNA methylation is crucial for cellular function and development.
- Profiling DNA methylation at single-cell resolution is essential for understanding cellular heterogeneity.
- Existing methods may be limited in throughput or cell type applicability.
Purpose of the Study:
- To present a standardized 96-well plate protocol for single-cell bisulfite sequencing (scBS-seq).
- To enable high-resolution DNA methylation profiling across diverse cell types.
- To facilitate studies on methylation in rare cell populations.
Main Methods:
- Detailed protocol for single-cell bisulfite sequencing (scBS-seq).
- Adaptation for a 96-well plate format for moderate throughput.
- Application to mammalian oocytes and early embryonic cells.
Main Results:
- Successful implementation of scBS-seq at single-nucleotide resolution.
- Demonstrated utility in profiling methylation in rare cell types.
- Established a scalable protocol for epigenomic studies.
Conclusions:
- The 96-well plate scBS-seq protocol is a valuable tool for epigenomic research.
- This method allows for detailed analysis of DNA methylation in challenging cell types.
- Facilitates deeper understanding of cell-to-cell epigenetic variation.
