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Long non‑coding RNA MCM3AP‑AS1 drives ovarian cancer progression via the microRNA‑143‑3p/TAK1 axis

Jihong Wen1, Shumei Han1, Man Cui1

  • 1Department of Gynecology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.

Oncology Reports
|September 18, 2020
PubMed

Insights

The long non-coding RNA MCM3AP-AS1 is elevated in ovarian cancer (OC) and promotes tumor growth by interacting with miR-143-3p. This interaction upregulates TAK1, suggesting MCM3AP-AS1 as a potential therapeutic target for OC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer.
  • The specific role of MCM3AP antisense 1 (MCM3AP-AS1) in ovarian cancer (OC) remains unclear.
  • Previous studies implicated MCM3AP-AS1 in various cancers, necessitating investigation in OC.

Purpose of the Study:

  • To investigate the expression and function of MCM3AP-AS1 in ovarian cancer.
  • To elucidate the molecular mechanism underlying MCM3AP-AS1's role in OC progression.
  • To assess the potential of MCM3AP-AS1 as a therapeutic target in OC.

Main Methods:

  • Quantitative real-time PCR to measure MCM3AP-AS1 expression in OC tissues.
  • In vitro assays (cell proliferation, migration, colony formation) following MCM3AP-AS1 knockdown.
  • In vivo tumor growth assays in a mouse model.
  • RNA immunoprecipitation and luciferase reporter assays to confirm MCM3AP-AS1 interaction with miR-143-3p.
  • Western blotting to assess TAK1 expression.

Main Results:

  • MCM3AP-AS1 expression was significantly upregulated in OC tissues and correlated with advanced tumor stage, lymph node metastasis, and poorer survival.
  • Knockdown of MCM3AP-AS1 inhibited OC cell proliferation, migration, colony formation, and tumor growth in vivo.
  • MCM3AP-AS1 directly binds to miR-143-3p, acting as a competing endogenous RNA (ceRNA).
  • This interaction leads to increased expression of transforming growth factor-β-activated kinase 1 (TAK1) in OC cells.
  • Inhibition of miR-143-3p partially reversed the effects of MCM3AP-AS1 knockdown on OC cell proliferation, migration, and invasion.

Conclusions:

  • MCM3AP-AS1 acts as an oncogenic lncRNA in ovarian cancer.
  • It promotes OC progression by sponging miR-143-3p and subsequently increasing TAK1 expression.
  • MCM3AP-AS1 represents a promising therapeutic target for ovarian cancer treatment.

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