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A whole blood microsampling assay for vancomycin: development, validation and application for pediatric clinical
Ganesh S Moorthy1,2, Kevin J Downes1,3, Christina Vedar1
1Center for Clinical Pharmacology, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
Bioanalysis
|September 18, 2020
Summary
This study developed a validated LC-MS/MS method to quantify vancomycin in dried blood microsamples using volumetric absorptive microsampling (VAMS). This approach simplifies therapeutic drug monitoring for vancomycin in pediatric studies.
Area of Science:
- Pharmacology
- Analytical Chemistry
- Clinical Research
Background:
- Vancomycin is a critical antibiotic requiring therapeutic drug monitoring for efficacy.
- Microsampling assays offer advantages for pediatric clinical research and drug level monitoring.
Purpose of the Study:
- To develop and validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying vancomycin.
- To assess the stability of vancomycin in volumetric absorptive microsampling (VAMS) devices.
Main Methods:
- Quantification of vancomycin in human whole blood using 20 μl VAMS devices.
- Validation of the LC-MS/MS assay for accuracy and precision.
- Stability testing of dried vancomycin microsamples under specific storage conditions.
Main Results:
- A robust LC-MS/MS method for vancomycin quantification in VAMS was successfully developed and validated.
- Vancomycin demonstrated stability in dried whole blood microsamples for at least 160 days at -78°C.
- The method is currently employed in an ongoing pediatric clinical study.
Conclusions:
- The validated LC-MS/MS method using VAMS is suitable for vancomycin therapeutic drug monitoring in pediatric populations.
- This microsampling technique facilitates clinical studies by simplifying sample collection and handling.
- The stability data supports the use of dried VAMS for vancomycin analysis in research settings.
Keywords:
LC–MS/MShuman whole bloodpediatricstherapeutic drug monitoringvancomycinvolumetric absorptive microsamplingMore Related Videos
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