Related Experiment Videos
Functionally involved cell surface antigens on murine lymphokine activated killer cells
Immunology Letters
|April 1, 1987
Summary
Investigating lymphokine-activated killer (LAK) cell activity, researchers found that antibodies targeting LFA-1 and Lyt 2 antigens significantly inhibited cytotoxicity. These cell surface structures are crucial for LAK cell function against specific tumor targets.
Area of Science:
- Immunology
- Cell Biology
Background:
- Lymphokine-activated killer (LAK) cells are crucial for cellular immunity.
- Understanding the role of cell surface structures on LAK cells is vital for immunotherapy.
Purpose of the Study:
- To identify specific cell surface molecules involved in LAK cell functional activity.
- To determine the inhibitory effects of monoclonal antibodies against these structures on LAK cell cytotoxicity.
Main Methods:
- Utilized monoclonal antibodies (Mab) against various lymphoid cell surface structures.
- Performed standard cytotoxicity assays using natural killer-insensitive EL-4 target cells.
- Investigated inhibition of LAK activity by preincubating effector and target cells separately.
Main Results:
- Antibodies to Lymphocyte Function-Associated Antigen 1 (LFA-1) almost completely inhibited LAK activity.
- Blocking of the Lyt 2 antigen reduced LAK cytotoxic activity by approximately 50%.
- Antibodies to Thy 1, L3T4, IL-2 receptor, and MHC class I antigens showed no significant effect on LAK activity.
Conclusions:
- LFA-1 and Lyt 2 antigens play significant roles in mediating LAK cell cytotoxicity.
- These inhibitory effects are primarily mediated at the effector cell level.
- LAK cell surface molecules LFA-1 and Lyt 2 are critical for targeting specific tumor cells.