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Updated: Dec 8, 2025

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
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Structural Studies of the Lipopolysaccharide Isolated from Plesiomonas shigelloides O22:H3 (CNCTC 90/89)

Anna Maciejewska1, Brygida Bednarczyk1, Czeslaw Lugowski1

  • 1Laboratory of Microbial Immunochemistry and Vaccines, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wroclaw, Poland.

International Journal of Molecular Sciences
|September 19, 2020
PubMed

Insights

Plesiomonas shigelloides lipopolysaccharide (LPS) structure was elucidated for O22. This research details the O-specific polysaccharide and core oligosaccharide structures, enhancing understanding of this bacterial virulence factor.

Area of Science:

  • Microbiology
  • Carbohydrate Chemistry
  • Structural Biology

Background:

  • Plesiomonas shigelloides causes foodborne infections and traveler's diarrhea.
  • Lipopolysaccharide (LPS) is a key virulence factor, but its structure is poorly characterized in relation to O-serotypes.
  • Only 15 of 102 O-serotypes of P. shigelloides LPS structures have been previously determined.

Purpose of the Study:

  • To elucidate the chemical structure of the O-specific polysaccharide and core oligosaccharide of Plesiomonas shigelloides O22.
  • To contribute to the understanding of P. shigelloides LPS structure-virulence relationships.
  • To expand the knowledge of characterized LPS structures for P. shigelloides.

Main Methods:

  • Chemical analysis
  • Mass spectrometry
  • Nuclear magnetic resonance (NMR) spectroscopy (¹H and ¹³C)

Main Results:

  • The O-specific polysaccharide repeating unit was determined to be a pentasaccharide.
  • The pentasaccharide contains one d-QuiNAc and four d-GalNAcAN residues.
  • The core oligosaccharide lacks phosphate groups and contains uronic acids, consistent with other P. shigelloides endotoxins.

Conclusions:

  • The detailed structure of P. shigelloides O22 LPS has been reported.
  • This structural information advances the characterization of P. shigelloides virulence factors.
  • The findings provide a basis for further investigation into the role of LPS structure in P. shigelloides pathogenesis.
Keywords:
LPSNMRO-specific polysaccharidecore oligosaccharidelipopolysaccharidemass spectrometry

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