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Metabolic Profiling of a Porcine Combat Trauma-Injury Model Using NMR and Multi-Mode LC-MS Metabolomics-A Preliminary
Anna Karen Carrasco Laserna1, Yiyang Lai2, Guihua Fang1,3
1Department of Chemistry, Faculty of Science, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.
Metabolites
|September 19, 2020
Summary
Combat trauma causes severe bleeding and shock, leading to organ failure. This study identified key metabolic changes in a porcine model, offering insights into potential biomarkers for diagnosing hemorrhagic shock and organ dysfunction.
Area of Science:
- Biochemistry
- Trauma Medicine
- Metabolomics
Background:
- Penetrating trauma and associated hemorrhage are common in combat, often leading to trauma hemorrhagic shock (THS).
- THS results in oxygen deficiency, metabolic derangements, and inflammation, potentially causing organ failure and death.
- Understanding acute metabolic changes post-THS is crucial for developing interventions and identifying diagnostic biomarkers.
Purpose of the Study:
- To investigate the acute metabolic alterations following penetrating trauma and hemorrhagic shock in a preclinical model.
- To identify potential biomarkers for early diagnosis of THS and subsequent organ dysfunction.
Main Methods:
- Utilized a porcine model simulating combat-related penetrating limb trauma and hemorrhagic shock.
- Employed a metabolomic approach combining Nuclear Magnetic Resonance (NMR) spectroscopy and Liquid Chromatography-Mass Spectrometry (LC-MS).
Main Results:
- Identified significant metabolic changes after a 30-minute shock period.
- Detected alterations in metabolites linked to acute-phase reactions, inflammation, energy depletion, oxidative stress, and potential renal dysfunction.
Conclusions:
- Metabolomic profiling reveals significant biochemical shifts in response to combat trauma and hemorrhagic shock.
- These findings provide a foundation for developing diagnostic tools and therapeutic strategies for THS patients.
