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LncRNA STARD13-AS blocks lung squamous carcinoma cells growth and movement by targeting miR-1248/C3A

Guosen Li1, Xiangyun Guo2

  • 1Queen Mary School of Medical College, Jiangxi Medical College, Qianhu Campus, Nanchang University, No. 1299 Xuefu Street, Nanchang, Jiangxi, China.

Pulmonary Pharmacology & Therapeutics
|September 19, 2020
PubMed
Abstract

Insights

Overexpression of long non-coding RNA STARD13-AS inhibits lung squamous cell carcinoma growth and metastasis by regulating the miR-1248/C3A axis. This finding offers a potential therapeutic target for LUSC. Keywords: STARD13-AS, miR-1248, C3A, lung squamous cell carcinoma, metastasis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Lung squamous cell carcinoma (LUSC) is a major subtype of lung cancer with complex molecular underpinnings.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development and progression.
  • The specific roles of lncRNA STARD13-AS, miR-1248, and C3A in LUSC remain to be fully elucidated.

Purpose of the Study:

  • To investigate the regulatory network involving lncRNA STARD13-AS, miR-1248, and C3A in lung squamous cell carcinoma.
  • To determine the effect of STARD13-AS on the biological functions of LUSC cells, including growth, invasion, and migration.
  • To explore the potential of the STARD13-AS/miR-1248/C3A axis as a therapeutic target for LUSC.

Main Methods:

  • Bioinformatic analysis of TCGA and GEPIA databases to assess STARD13-AS, miR-1248, and C3A expression in LUSC.
  • Construction of overexpression and knockdown models using cell transfection.
  • Quantitative PCR (qPCR) to confirm transfection efficiency.
  • In vitro assays including CCK-8, colony formation, and Transwell assays to evaluate cell growth, invasion, and migration.
  • Luciferase reporter assays to validate the interactions between STARD13-AS, miR-1248, and C3A.

Main Results:

  • C3A expression was significantly downregulated in LUSC tissues.
  • STARD13-AS was underexpressed in LUSC cells and negatively impacted cell growth.
  • miR-1248 was identified as a downstream target of STARD13-AS and an upstream regulator of C3A.
  • STARD13-AS was found to co-regulate C3A expression and modulate the effects of miR-1248 on LUSC cell proliferation, invasion, and migration.

Conclusions:

  • Overexpression of STARD13-AS effectively inhibited the growth and aggressiveness of LUSC cells.
  • The STARD13-AS/miR-1248/C3A axis plays a crucial role in LUSC progression.
  • Targeting the STARD13-AS/miR-1248/C3A pathway represents a promising therapeutic strategy for LUSC.
Keywords:
InvasionLung squamous carcinomaMigrationProliferationceRNA

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