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Human neutrophils compromise the restoration-tooth interface
Russel Gitalis1, Ji Hyeon Bae2, Matthew Preston1
1Faculty of Dentistry, University of Toronto, Toronto, ON, Canada; Institute of Biomedical Engineering, University of Toronto, Toronto, ON, Canada.
Acta Biomaterialia
|September 20, 2020
Summary
Neutrophils degrade dental resin composites and adhesives, reducing the bond strength at the tooth interface. This immune cell activity may contribute to dental caries and restoration failure.
Area of Science:
- Biomaterials Science
- Immunology
- Dental Materials
Background:
- Neutrophils, key innate immune cells, infiltrate oral tissues.
- Their role in degrading dental restorative materials and tooth dentin is hypothesized.
- Understanding this degradation is crucial for improving dental restoration longevity.
Purpose of the Study:
- To characterize neutrophil-mediated degradation of resin composites and adhesives.
- To evaluate the impact of neutrophils on resin-dentin interfaces.
- To identify specific proteins involved in neutrophil-induced degradation.
Main Methods:
- Quantified resin degradation by measuring bishydroxy-propoxy-phenyl-propane (bisHPPP) release.
- Assessed interfacial fracture toughness (FT) of resin-dentin interfaces.
- Analyzed fracture modes of SE and TE resin-dentin interfaces after neutrophil incubation.
Main Results:
- Neutrophils increased degradation of resin composite and total-etch (TE) adhesives, but not self-etch (SE) adhesives.
- Interfacial fracture toughness of SE and TE resin-dentin interfaces was reduced by neutrophils, more significantly for TE.
- Neutrophils altered the fracture modes of both SE and TE resin-dentin interfaces.
Conclusions:
- Neutrophils degrade common dental restorative materials and compromise the integrity of resin-dentin bonds.
- Proteins like MMP-8, MMP-9, Cathepsin G, NGAL, and Myeloperoxidase contribute to this degradation.
- Neutrophil activity may play a significant role in the development of dental caries and failure of dental restorations.
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