Interaction of BSA with ZnO, TiO₂, and CeO₂ nanoparticles

This article aims to explore interaction of nanoparticles ZnO, TiO₂ and CeO₂ with bovine serum albumin (BSA). The samples of nanoparticles were laboratory prepared and compared with commercial nanoparticles. Nanoparticles were characterized by SEM, zeta potential and size measurements. Adsorption of particles took place at pH where the zeta potential of the protein and nanoparticles was opposite. Then the zeta potential and size distribution were measured and the amount of protein needed to reduce the zeta potential of nanoparticles to zero was determined. The changes of BSA structure were also observed by Circular Dichroism (CD). The change of BSA structure after the adsorption on nanoparticles was confirmed. The content of BSA α-helix structure varies during experiments from 13 to 77% in dependence of concentration of nanoparticles. The interaction of TiO₂ and BSA was confirmed also by the Surface Plasmon Resonance (SPR) technique. On laboratory prepared TiO₂ was bound amount of 73,4 pg·mm^-2.