New multimodal stationary phases prepared by Ugi multicomponent approach

Area of Science:

• Analytical Chemistry
• Materials Science

Background:

• Developing versatile stationary phases is crucial for advancing multimodal chromatography.
• The Ugi reaction offers a flexible platform for synthesizing diverse chemical structures.

Purpose of the Study:

• To synthesize and characterize novel stationary phases for multimodal chromatography using the Ugi reaction.
• To demonstrate the versatility of the Ugi synthetic protocol in creating sorbents with distinct properties.
• To evaluate the chromatographic performance of the synthesized phases in reversed-phase, hydrophilic, and ion-exchange modes.

Main Methods:

• Preparation of eight stationary phases via a four-component Ugi reaction on two different aminopropyl silicas.
• Immobilization of diverse functional groups including aliphatic chains, phenyl, cholic acid, phenylboronic acid, monosaccharide, betaine, and arginine moieties.
• Characterization using elemental analysis, Raman spectroscopy, and chromatographic evaluation.

Main Results:

• The Ugi reaction successfully generated stationary phases with significantly varied hydrophobicity and ion-exchange capabilities.
• Chromatographic evaluation confirmed the potential for reversed-phase, hydrophilic, and ion-exchange separations.
• The choice of the underlying aminopropyl silica influenced the final chromatographic behavior of the stationary phases.
• Successful separation of cytarabine demonstrated the practical applicability of the novel sorbents.

Conclusions:

• The Ugi synthetic protocol is a simple, feasible, and versatile method for creating chromatography sorbents with tunable properties.
• The synthesized stationary phases exhibit diverse characteristics suitable for multimodal chromatographic applications.
• The developed stationary phases show promise for practical applications in chemical separations, such as the analysis of cytarabine.