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Preparation and Characterization of Individual and Multi-drug Loaded Physically Entrapped Polymeric Micelles
Published on: August 28, 2015
Preparation of Poly(MTZ) -(DMAEMA) Micelles and Study on Their Antibacterial Property
Long Zhang1, Yongkang Zhang1, Gang Zhao1
1Insititute of Nervous System Diseases, Xuzhou Medical University, Xuzhou 221002, P. R. China.
Abstract:
Bacterial infections are the most common type of clinical infection. The abuse of clinical antibiotics has led to the frequent appearance of drug-resistant strains and even some super bacteria. In this study, we synthesized Poly(MTZ) -(DMAEMA) polymer micelles with cations on the surface. The synthesis of this novel polymer comes in two steps. First, Poly(MTZ) was synthesized with metronidazole (MTZ) referred as the macromolecular chain transfer agent and v-501 as the initiator for initiating the polymerization of 4-cyanopentanoic acid dithiobenzoate. Then, novel polymer micelles were synthesized with Poly(MTZ) referred as the macromolecular chain transfer agent and v-501 as the initiator for initiating the polymerization of the monomer 2-(dimethylamino) ethyl methacrylate, which could adsorb to the negatively charged bacterial surface via electrostatic interaction and enhance bactericidal activity. Scanning electron microscopy showed that the micelles could be accurately targeted to the surface of bacteria, and the zone of inhibition assay confirmed that the micelles could enhance the sensitivity of bacteria to drugs. Hence, Poly(MTZ) -(DMAEMA) polymer micelles will have potential use for the clinical treatment of anaerobic infections in the future.
Insights
Novel polymer micelles targeting bacteria were developed to combat antibiotic resistance. These Poly(MTZ)-(DMAEMA) micelles enhance drug sensitivity, offering a potential solution for treating drug-resistant bacterial infections.
Area of Science:
- Polymer Chemistry
- Antimicrobial Research
- Nanotechnology
Background:
- Clinical antibiotic abuse fuels the rise of drug-resistant bacteria and superbugs.
- Bacterial infections are a significant global health concern, necessitating novel therapeutic strategies.
- Developing effective treatments against resistant bacterial strains is crucial for public health.
Purpose of the Study:
- To synthesize novel Poly(MTZ)-(DMAEMA) polymer micelles with surface cations.
- To investigate the targeted delivery and enhanced bactericidal activity of these polymer micelles.
- To evaluate the potential of these micelles in overcoming antibiotic resistance.
Main Methods:
- Two-step polymer synthesis involving metronidazole (MTZ) and 2-(dimethylamino) ethyl methacrylate (DMAEMA).
- Utilizing scanning electron microscopy (SEM) for visualizing micelle targeting to bacterial surfaces.
- Employing zone of inhibition assays to assess enhanced drug sensitivity.
Main Results:
- Poly(MTZ)-(DMAEMA) polymer micelles were successfully synthesized with cationic surfaces.
- SEM confirmed accurate targeting of micelles to bacterial surfaces.
- Zone of inhibition assays demonstrated enhanced bacterial sensitivity to drugs when treated with the micelles.
Conclusions:
- The synthesized Poly(MTZ)-(DMAEMA) polymer micelles show potential for targeted delivery and enhanced antimicrobial efficacy.
- These micelles offer a promising strategy to combat antibiotic-resistant bacteria, particularly in anaerobic infections.
- Further clinical applications for treating infections caused by drug-resistant bacteria are anticipated.
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