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"Plug-n-Play" Polymer Substrates: Surface Patterning with Reactive-Group-Appended Poly-l-lysine for Biomolecule
Jacopo Movilli1, Daniele Di Iorio1, Andrea Rozzi2
1Molecular Nanofabrication Group, MESA+ Institute for Nanotechnology, Department of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
ACS Applied Polymer Materials
|September 21, 2020
Summary
Modified poly-L-lysine (PLL) enables selective biomolecule immobilization on various polymers for biosensing. This method ensures stable, patterned surfaces capable of specific DNA detection, advancing biomedical device fabrication.
Area of Science:
- Biomaterials Science
- Surface Chemistry
- Nanotechnology
Background:
- Immobilizing biomolecules on polymers for biomedical devices is difficult due to limited functional groups.
- Existing surface chemistries are often incompatible with common polymeric materials.
Purpose of the Study:
- To develop a versatile method for selective biomolecule immobilization on challenging polymeric surfaces.
- To create stable, patterned surfaces for biosensing applications.
Main Methods:
- Utilized modified poly-L-lysine (PLL) with biotin or maleimide functional groups.
- Employed micromolding in capillaries (MIMIC) and hydrogel stamping for microscale patterning.
- Verified surface modification and stability using fluorescence microscopy, contact angle, and XPS.
Main Results:
- Achieved stable surface functionalization on Cyclic Olefin Polymer (COP), Ormostamp, and Polydimethylsiloxane (PDMS) for over 20 days.
- Successfully immobilized DNA and PNA probes via biotin-streptavidin or thiol-maleimide coupling.
- Demonstrated specific DNA sequence recognition and multiplexed detection using PNA microarrays.
Conclusions:
- Modified PLL provides an effective strategy for functionalizing diverse polymer surfaces with biomolecules.
- The developed method supports stable microscale patterning and specific biomolecular interactions for biosensing.
- This approach holds potential for advanced multiplexed DNA detection in biomedical applications.
