Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bimodality in pan-cancer proteomics reveals new opportunities for biomarker discovery.

Genome biology·2026
Same author

Endothelial cannabinoid CB1 receptor deficiency reduces shear stress-induced arterial inflammation and lipid uptake.

Nature communications·2026
Same author

Study protocol: double-blind, randomized, prospective, placebo controlled parallel group phase II study to investigate the effect of glycerol phenylbutyrate (GPB) on neurofilament light chain (NfL) levels in patients with corticobasal syndrome (CBS).

Neurological research and practice·2026
Same author

Multiple adhesion molecules act together in oligodendrocyte-mediated axonal selection and myelin formation.

PLoS biology·2026
Same author

From mechanism to substratome: unraveling mysteries of γ-secretase.

The Journal of biological chemistry·2026
Same author

Aberrant immunomodulatory signature in β-propeller protein-associated neurodegeneration patient iPSC-derived microglia.

Scientific reports·2026

Related Experiment Video

Updated: Dec 8, 2025

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning
10:36

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning

Published on: December 15, 2016

10.9K

An optimized quantitative proteomics method establishes the cell type-resolved mouse brain secretome.

Johanna Tüshaus1,2, Stephan A Müller1,2, Evans Sioma Kataka3

  • 1German Center for Neurodegenerative Diseases (DZNE), Munich, Germany.

The EMBO Journal
|September 21, 2020
PubMed
Summary

We developed a new method, high-performance secretome protein enrichment with click sugars (hiSPECS), to analyze cell communication in the brain. This technique helps identify brain secretome proteins and their cellular origins, aiding in disease biomarker discovery.

Keywords:
CSFBACE1brain cellsproteomicssecretomics

More Related Videos

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

4.9K
Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis
06:24

Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis

Published on: October 7, 2021

3.7K

Related Experiment Videos

Last Updated: Dec 8, 2025

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning
10:36

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning

Published on: December 15, 2016

10.9K
Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

4.9K
Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis
06:24

Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis

Published on: October 7, 2021

3.7K

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Understanding cellular communication in the nervous system requires defining the secretome.
  • Analyzing the secretome of primary cells is challenging due to the large cell numbers needed.

Purpose of the Study:

  • To miniaturize secretome analysis for primary cells.
  • To develop a novel method for high-performance secretome protein enrichment.
  • To create a cell type-resolved mouse brain secretome resource.

Main Methods:

  • Developed the "high-performance secretome protein enrichment with click sugars" (hiSPECS) method.
  • Applied hiSPECS to analyze the secretory response of brain slices to LPS-induced neuroinflammation.
  • Used hiSPECS to establish a cell type-resolved mouse brain secretome resource from primary astrocytes, microglia, neurons, and oligodendrocytes.

Main Results:

  • hiSPECS successfully miniaturized secretome analysis.
  • The study identified the secretory response of brain slices during neuroinflammation.
  • A comprehensive mouse brain secretome resource was established, revealing cell type-specific secretion.
  • Discovered that many secreted proteins are cleaved ectodomains of membrane proteins.
  • Identified neuronally secreted ADAM22 and CD200 as substrates of the Alzheimer's-associated protease BACE1.

Conclusions:

  • hiSPECS is a broadly applicable method for studying protein secretion.
  • The brain secretome resource enables mapping of cellular protein origins.
  • The findings highlight the significance of cleaved membrane protein ectodomains in secretion.
  • This resource and method can advance the study of brain function and identify CNS disease biomarkers.