A review: Red/near-infrared (NIR) fluorescent probes based on nucleophilic reactions of H₂ S since 2015

The topics of human health and disease are always the focus of much attention. Hydrogen sulfide (H₂ S), as a double-edged sword, plays an important role in biological systems. Studies have revealed that endogenous H₂ S is important to maintain normal physiological functions. Conversely, abnormal levels of H₂ S may contribute to various diseases. Due to the importance of H₂ S in physiology and pathology, research into the effects of H₂ S has been active in recent years. Fluorescent probes with red/near-infrared (NIR) emissions (620-900 nm) are more suitable for imaging applications in vivo, because of their negligible photodamage, deep tissue penetration, and maximum lack of interference from background autofluorescence. H₂ S, an 'evil and positive' molecule, is not only toxic, but also produces significant effects; a 'greedy' molecule, is not only a strong nucleophile under physiological conditions, but also undergoes a continuous double nucleophilic reaction. Therefore, in this tutorial review, we will highlight recent advances made since 2015 in the development and application of red/NIR fluorescent probes based on nucleophilic reactions of H₂ S.