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InteBac: An integrated bacterial and baculovirus expression vector suite.

Veronika Altmannova1, Andreas Blaha1, Susanne Astrinidis1

  • 1Friedrich Miescher Laboratory of the Max-Planck-Society, Max-Planck-Ring 9, Tübingen, Germany.

Protein Science : a Publication of the Protein Society
|September 21, 2020
PubMed
Summary

This study introduces InteBac, an integrated cloning system for simultaneous expression in Escherichia coli and baculovirus systems. It streamlines recombinant protein production and screening, saving time and resources.

Keywords:
Gibson assemblybacterial expressioncloning systeminsect cell expressionpolycistronic

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Area of Science:

  • Molecular Biology
  • Protein Expression
  • Structural Biology

Background:

  • Recombinant protein production for structural studies relies heavily on expression organism choice.
  • Escherichia coli and baculovirus/insect cell systems are common, but baculovirus is costly and time-consuming.
  • Current methods often require re-cloning for different expression systems.

Purpose of the Study:

  • To develop an integrated system for simultaneous cloning into E. coli and baculovirus vectors.
  • To streamline the process of recombinant protein expression and screening.
  • To facilitate rapid generation of polycistronic constructs for protein complexes.

Main Methods:

  • Utilizing the same PCR products for cloning into both E. coli and baculovirus vectors.
  • Implementing a flexible array of N- and C-terminal affinity, solubilization, and utility tags.
  • Adapting the biGBac system for rapid generation of polycistronic bacterial constructs.

Main Results:

  • Successful simultaneous cloning into E. coli and baculovirus vectors.
  • Enabled rapid expression screening in E. coli prior to baculovirus experiments.
  • Facilitated the creation of polycistronic bacterial constructs.

Conclusions:

  • The InteBac system significantly accelerates and simplifies recombinant protein production workflows.
  • It offers a cost-effective and time-efficient alternative for structural biology studies.
  • InteBac is particularly beneficial for researchers studying recombinant protein complexes.