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Methylation of MMP2, TIMP2, MMP9 and TIMP1 in abdominal aortic aneurysm

M Skorvanova, T Matakova, M Skerenova

    Bratislavske Lekarske Listy
    |September 21, 2020
    PubMed
    Abstract

    Insights

    This pilot study investigated DNA methylation in matrix metalloproteinases and tissue inhibitors in abdominal aortic aneurysm (AAA) patients. No association was found between gene promoter methylation and AAA development.

    Area of Science:

    • Cardiovascular Diseases
    • Molecular Biology
    • Genetics

    Background:

    • Abdominal aortic aneurysm (AAA) is a serious cardiovascular condition with increasing incidence.
    • Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) play roles in vascular remodeling.
    • Aberrant DNA methylation of MMP and TIMP genes is implicated in various diseases.

    Purpose of the Study:

    • To explore the relationship between DNA methylation in the promoter regions of MMP2, TIMP2, TIMP1, and MMP9 genes and the development of AAA.
    • To assess the methylation status of these key genes in peripheral blood of AAA patients and healthy controls.

    Main Methods:

    • A case-control study involving 43 males with AAA and 34 healthy males.
    • Methylation-specific polymerase chain reaction (MS-PCR) was used to detect promoter methylation status of MMP2, TIMP2, TIMP1, and MMP9 genes.

    Main Results:

    • No significant differences in methylation patterns were observed for MMP2, TIMP2, and TIMP1 between AAA patients and controls.
    • High methylation ratios for MMP9 were found in both AAA patients (93.0%) and controls (91.2%), indicating no specific association with AAA.

    Conclusions:

    • This pilot study found no association between the DNA methylation of MMP2, TIMP2, TIMP1, and MMP9 genes and the development of abdominal aortic aneurysm.
    • Further research with larger cohorts may be warranted to fully elucidate the role of gene methylation in AAA pathogenesis.
    Keywords:
    DNA methylation methylation status.abdominal aortic aneurysmmatrix metalloproteinase

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