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LncRNA AFAP1-AS1 Knockdown Represses Cell Proliferation, Migration, and Induced Apoptosis in Breast Cancer by

Bo Cai1, Xichao Wang1, Qing'ao Bu1

  • 1Department of Thyroid Surgery & Ward Area of Breast Surgery, Shengli Oilfield Central Hospital, Dongying, China.

Cancer Biotherapy & Radiopharmaceuticals
|September 21, 2020
PubMed

Insights

Long non-coding RNA AFAP1-AS1 promotes breast cancer progression by upregulating SEPT2 via sponging miR-497-5p. Knocking down AFAP1-AS1 inhibits tumor growth, migration, and enhances apoptosis in breast cancer cells.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNA actin filament-associated protein1-antisense RNA 1 (AFAP1-AS1) is implicated in tumorigenesis.
  • The specific role of AFAP1-AS1 in breast cancer remains largely uncharacterized.

Purpose of the Study:

  • To investigate the function of AFAP1-AS1 in breast cancer.
  • To elucidate the molecular mechanism involving AFAP1-AS1, microRNA-497-5p (miR-497-5p), and Septin 2 (SEPT2) in breast cancer progression.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) for gene expression analysis.
  • Cell proliferation, migration, and apoptosis assays (MTT, Transwell, Flow cytometry).
  • Dual-luciferase reporter assay, Western blot, and xenograft models for mechanistic and in vivo validation.

Main Results:

  • AFAP1-AS1 was significantly upregulated in breast cancer tissues and cells.
  • AFAP1-AS1 knockdown suppressed proliferation and migration, while promoting apoptosis.
  • AFAP1-AS1 acts as a sponge for miR-497-5p, leading to upregulation of its target gene, SEPT2.

Conclusions:

  • AFAP1-AS1 promotes breast cancer progression by regulating the miR-497-5p/SEPT2 axis.
  • Targeting AFAP1-AS1 may represent a potential therapeutic strategy for breast cancer.
Keywords:
AFAP1-AS1SEPT2breast cancermiR-497-5pproliferation

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