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The Class A Carbapenemases BKC-1 and GPC-1 Both Originate from the Bacterial Genus Shinella
Nicolas Kieffer1,2, Stefan Ebmeyer1,2, D G Joakim Larsson3,2
1Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, Gothenburg, Sweden.
Abstract:
Comparative genomics identified the environmental bacterial genus Shinella as the most likely origin of the class A carbapenemases BKC-1 and GPC-1. Available sequences and PCR analyses of additional Shinella species revealed homologous β-lactamases showing up to 85.4% and 93.3% amino acid identity to both enzymes, respectively. The genes conferred resistance to β-lactams once expressed in Escherichia coliblaBKC-1 likely evolved from a putative ancestral Shinella gene with higher homology through duplication of a gene fragment.
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