Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Upstream Processing

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...

Production of Biopesticides

Production of Biopesticides

Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...

Vaccine Production

Vaccine Production

Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Characterization and evaluation of a novel polylactic acid-made bioreactor for large-scale adherent cell expansion.

Applied microbiology and biotechnology·2026

Same author

Fouling Control of Ion-Selective Electrodes (ISEs) in Aquatic and Aquacultural Environments: A Comprehensive Review.

Sensors (Basel, Switzerland)·2025

Same author

<i>Pleurotus djamor</i> Mycelium: Sustainable Production of a Promising Protein Source from Carrot Side Streams.

Journal of agricultural and food chemistry·2025

Same author

Epigenetic silencing and CRISPR-mediated reactivation of tight junction protein claudin10b (CLDN10B) in renal cancer.

Clinical epigenetics·2025

Same author

Efficient Separation of a Novel Microbial Chassis, <i>Vibrio natriegens</i>, from High-Salt Culture Broth Using Ceramic Ultrafiltration Membranes.

Membranes·2025

Same author

Production of Oncolytic Measles Virus in Vero Cells: Impact of Culture Medium and Multiplicity of Infection.

Viruses·2024

Search research articles

Related Experiment Video

Updated: Jul 13, 2026

Production and Purification of Baculovirus for Gene Therapy Application

Production and Purification of Baculovirus for Gene Therapy Application

Published on: April 9, 2018

Bioreactor-Based Antigen Production Process Using the Baculovirus Expression Vector System.

Julie Harnischfeger¹, Lukas Käßer¹, Jan Zitzmann¹

¹Institute of Bioprocess Engineering and Pharmaceutical Technology (IBPT), Technische Hochschule Mittelhessen (THM) - University of Applied Sciences, Giessen, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|September 22, 2020

Summary

The baculovirus expression vector system (BEVS) is used to create protein-based vaccines. This chapter details methods for generating recombinant DNA, cultivating cells, and producing vaccines using BEVS in bioreactors.

Keywords:

Antigen production Cell cultivation Dielectric spectroscopy Online process monitoring Process analytical technology Sf-9 cells Spodoptera frugiperda

More Related Videos

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Published on: August 20, 2018

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Published on: July 17, 2021

Related Experiment Videos

Last Updated: Jul 13, 2026

Production and Purification of Baculovirus for Gene Therapy Application

Production and Purification of Baculovirus for Gene Therapy Application

Published on: April 9, 2018

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Published on: August 20, 2018

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Published on: July 17, 2021

Area of Science:

Biotechnology
Molecular Biology
Vaccine Development

Background:

The baculovirus expression vector system (BEVS) is a powerful tool for producing recombinant proteins.
Several vaccines are currently manufactured using BEVS, highlighting its industrial relevance.

Purpose of the Study:

To describe methods for generating recombinant baculoviral DNA (bacmid).
To outline procedures for cultivating Spodoptera frugiperda Sf-9 cells.
To detail the production of baculovirus stocks and protein-based vaccines using BEVS in stirred tank reactors.

Main Methods:

Recombinant bacmid DNA generation.
Cultivation of Spodoptera frugiperda Sf-9 insect cells.
Baculovirus stock production.
Protein-based vaccine production in stirred tank reactors using BEVS.

Main Results:

Successful generation of recombinant bacmid DNA.
Establishment of protocols for Sf-9 cell cultivation and baculovirus stock production.
Demonstration of protein-based vaccine production via BEVS in a bioreactor setting.

Conclusions:

The described methods provide a comprehensive workflow for vaccine production using BEVS.
This chapter serves as a practical guide for researchers and manufacturers utilizing BEVS for vaccine development.