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Production and Purification of Baculovirus for Gene Therapy Application
Published on: April 9, 2018
Bioreactor-Based Antigen Production Process Using the Baculovirus Expression Vector System
Julie Harnischfeger1, Lukas Käßer1, Jan Zitzmann1
1Institute of Bioprocess Engineering and Pharmaceutical Technology (IBPT), Technische Hochschule Mittelhessen (THM) - University of Applied Sciences, Giessen, Germany.
Abstract:
Several vaccines are already produced using the baculovirus expression vector system (BEVS). This chapter describes methods for generating recombinant baculoviral DNA (also called bacmid) for cultivating Spodoptera frugiperda Sf-9 cells and producing a baculovirus stock from the recombinant bacmid and for producing a protein-based vaccine with the BEVS in a stirred tank reactor.
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