Liposomal Dual Delivery of Both Polysaccharide and Protein Antigens

Pneumococcal disease is caused by Streptococcus pneumoniae, a colonizing microorganism characterized by transitioning from a benign commensal to a virulent pathogen in the presence of suitable circumstances, which then poses a serious infectious disease threat afflicting millions of people. Especially affected are the young and elderly through outcomes that include pneumonia, bacteremia, meningitis, and otitis media. Current prevention vaccine options on the market contain capsular polysaccharides conjugated to the Diphtheria CRM197 protein (Pfizer) or are composed of only pneumococcal polysaccharides (Merck), and in both cases, limitations prevent the generation of comprehensive disease protection. Through the use of a liposomal carrier, we present an alternative methodology for producing a vaccine product via noncovalent colocalization of both polysaccharide and protein classes of complementary pneumococcal disease immunogens.